Q: Describe the principle of ELISA
A: (Since what is IF is not clearly mentioned question; thus we have answered ELISA principle below.)…
Q: What is the purpose of an agglutination reaction test?
A: Antigen are the foreign substances to the body in the form of proteins, carbohydrates which may…
Q: What test requires inoculation of rabbit serum?
A: The formation of clot in the plasma indicates coagulase production. Coagulase is a protein enzymes…
Q: Explain how labeled antibodies are used in direct and indirect tests.
A: Antibodies or immunoglobulins are the primary line of defense in the body. They recognize and bind…
Q: Why do you coat the elisa plate with albumin?
A: ELISA It is enzyme linked immunosorbent assay. It is a common and powerful technique used for…
Q: Why is modified Allen test performed prior to the radial blood gas test?
A: Blood is an important fluid tissue that is used to transport nutrients and oxygen to all parts of…
Q: What are the advantages and disadvantages of agglutinationtests versus fluorescent antibody assays?…
A: Introduction The main property of our immune cells is that of specificity. They are highly specific…
Q: Which tests would be faster to perform? PCR or an ELISA test?
A: ELISA ELISA is enzyme linked immunosorbent assay. It is based upon antibody-antigen interaction.…
Q: What is the role of horseradish peroxidase conjugate in the ELISA experiment?
A: Horseradish peroxidase The enzyme horseradish peroxidase (HRP), located in horseradish's roots, is…
Q: What are the different technical considerations in performing the serum protein electrophoresis…
A: Electrophoresis is valuable as diagnostic tool in clinical laboratory. The principle involves the…
Q: What is Clinical test?
A: A disorder of structure or function in any living organism is called disease. This generally…
Q: What are the different solid phases that antibody or antigen can bind to, in ELISA?
A: Quantitative immunological techniques that use a solid phase can be defined as solid-phase…
Q: When is the RSBI measurement taken?
A: BASIC INFORMATION RSBI it stands for Rapid shallow breathing index it is basically the ratio of…
Q: An advantage of a point-of-care (POC) test is that
A: An advantage of a point of care (POC) is that effective antibiotic therapy can begin without a…
Q: Why does the antibody titer determination use twofold dilutions ofthe antiserum rather than 10-fold…
A:
Q: If an increase in CRP is nonspecific, what is the purpose of performing such a test, and how could…
A: CRP Test is known as the C-reactive protein test, which is used to determine inflammation.…
Q: What are the advantages and disadvantages of the viable plate count method?
A: Microbiology is the branch of science that studies microscopic organisms. As microorganisms are not…
Q: Describe how an ELISA test is performed, how the test works and provide examples of ELISA tests.
A: ELISA is an Enzyme-Linked Immunosorbent Assay, which is used to detect antibodies or antigens in the…
Q: how does teh ELISA help determined who the initial disease carrier is.
A: The enzyme linked immunosorbent assay is an analytical biochemistry assay which uses a solid phase…
Q: Describe the biuret test. What does the Biuret test detect?
A: There are different types of biomolecules. The biomolecules greatly differ in their physical and…
Q: Describe the Xanthroproteic test. What does the Xanthroproteic test detect?
A: Proteins are one among different biomolecules present in the body, and they are the building blocks…
Q: what are the important principle in invertase test?
A: To determine enzymatic activity, the amount of substrate remaining or the amount of product created…
Q: How does plating efficiency affect the number of plaque-forming units? How is plating efficiency…
A: A viral plaque is defined as the visible structure formed on the culture plate of bacteria or any…
Q: How do you measure antibiotic sensitivity?
A: Antibiotic susceptibility testing is the measurement of the sensitivity or resistance of a bacteria…
Q: What are the examples of organisms that belong to the positive test in in-dose test?
A: Tryptophan is an amino acid that can undergo deamination. Indole is generated by reductive…
Q: What are the advantages and disadvantages of using the E-test?
A: The E-test was created to give a direct measurement of microorganism antibiotic susceptibility. The…
Q: Name two types of immunodiffusion tests and describe how they operate
A: Immunodiffusion is a test which involves the use of soft agar or agarose to allow diffusion of…
Q: if you are running quantitative test where serum is diluted 6 times and you observed agglutination…
A: Agglutination is the mechanism by which antibodies pile together all the cells or particles like…
Q: What types of controls are used for the slide agglutination?
A: A protein produced by the immune system of body when it detects harmful substances is called…
Q: What advantages does the agglutination test have over the definitive S. aureus test?
A: Agglutination test helps to detect an antigen or antibody and involves agglutination of the…
Q: What does the direct ELISA test for?
A: ELISA(enzyme-linked immunosorbent assay) is a biochemical assay to detect the presence of ligand…
Q: What might cause a false positive presumptive test?
A: In the presumptive analysis, test tubes containing lactose broth are inoculated with an even…
Q: What is the proper way of Modified Allen test?
A: Allen test are the tests used to assess the arterial blood supply of the hands. This test is used…
Q: Give the full form of ELISA.which disease can be detected using it?Discuss the principle underlying…
A: It is a diagnostic tool for detecting substance like antigen.The rDNA technology has enabled the…
Q: What is the basis of serological tests, and what is their main use forenterics?
A: Serological tests are diagnostic procedures carried out to detect antibodies present in the blood…
Q: What two S. aureus antigens are being detected with the use of this test kit?
A: Antigen is a substance that is capable of stimulating an immune response. Specifically, it activates…
Q: Discuss the difference between nontreponemal agglutination test and treponemal agglutination test.
A: An antigen is a disease agent (virus, toxin, bacterial parasite, fungus, chemical, etc.) that our…
Q: in an indirect elisa procedure what enzyme is used?
A: Enzyme-linked immunosorbent assay (ELISA) is an assay technique used for detecting and quantifying…
Q: The direct ELISA test requires only one antibody only one antigen both primary and…
A: The direct ELISA test required only one antigen. In a direct ELISA an antigen is immobilized…
Q: what is the tuberculin test, and why is it used in clinics?
A: Tuberculin test It is a screening test for tuberculosis (TB). It is performed by injecting a small…
Q: For what ELISA is used?
A: A wide range of serological tests are available to recognize the antigens or antibodies in the…
Q: What is a false positive test result and what are some possible causes?
A: The abnormal condition in which the body is negatively affected is called the disease. The negative…
Q: The Xanthoproteic test involves what type of reaction?
A: The xanthoproteic reaction is the method that is used to detect a presence of the proteins soluble…
Q: What methods are used to identify the serovar when there is a Salmonella outbreak? What other…
A: Serovars can be defined as different strains or variants of a bacterial species or a virus that have…
Q: The test utilized for blood typing is?
A: Blood is a type of connective tissue. There are four types of blood group namely blood group A,B,O,…
Q: What does BCR ABL positive mean?
A: The cancer of the white blood cells is known as Chronic myelogenous leukemia (CML) in medical terms.…
Q: Describe how the direct and indirect ELISA are Different
A: The enzyme-linked immunosorbent assay (ELISA) is a biological experiment that measures antibodies,…
Q: Which portion of the blood is used for the Elisa test? Explain.
A: ELISA is an enzyme linked immunosorbent Assay which is a biochemical test used to detect the…
Q: Describe the mechanism of an Indirect ELISA. Why is ELISA so sensitive? Why is it necessary to block…
A: The enzyme linked immunosorbent assay (ELISA) is an analytical biochemistry assay which uses the…
How is the Elisa test quantified?
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