How does the spectrophotometer measure absorbance? Why does the dye absorb light (electron transitions)? What color light does Blue #1 dye absorb? What is the lambda max of the dye? What is Beer’s law and how can it be used to calculate the desired concentrations for your solutions? Considering Beer’s law, is it possible to look up the molar absorptivity coefficient of Blue #1 dye? How will you estimate what concentrations of dye will be required for an absorbance of 1.0 AU given a path length of 1.46cm? How would you dilute a 2.0 mM dye solution to make 100mL of this 1.0 AU solution? How can the 1.0 AU solution be diluted to form a point on the calibration curve at 0.25 AU?
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