Slide 8Please help me explain this on my report on cell dogma HOW DO WE KNOW THAT DNA REPLICATION ISSEMICONSERVATIVE?MESELSON-STAHL EXPERIMENTS15 N cells transferred to4 N mediumControl: E. coli grown for many 1. E. coli grown for manygenerations in r N medium.2. Cells replicate once toproduce first generationof daughter cells.3. Cells replicate a secondtime to produce a secondgeneration of daughter cells.30generations in 15 N medium.minutesExtract DNA from cells.Extract DNA from cells.Extract DNA from cells.Extract DNA from cells.CentrifugeCentrifugeCentrifugeCentrifugeDNA bands in cesium chloride gradient14N 14 N14N 14 N15 N 14 N15N 14 N15N 15 NResults confirm prediction of semiconservative replication.

DNA replication in all known cells is described as semiconservative replication. Along the DNA…

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Science

Biochemistry

HOW DO WE KNOW THAT DNA REPLICATION IS SEMICONSERVATIVE? MESELSON-STAHL EXPERIMENTS

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

Slide 8

Please help me explain this on my report on cell dogma

HOW DO WE KNOW THAT DNA REPLICATION IS
SEMICONSERVATIVE?
MESELSON-STAHL EXPERIMENTS
15 N cells transferred to4 N medium
Control: E. coli grown for many 1. E. coli grown for many
generations in r N medium.
2. Cells replicate once to
produce first generation
of daughter cells.
3. Cells replicate a second
time to produce a second
generation of daughter cells.
30
generations in 15 N medium.
minutes
Extract DNA from cells.
Extract DNA from cells.
Extract DNA from cells.
Extract DNA from cells.
Centrifuge
Centrifuge
Centrifuge
Centrifuge
DNA bands in cesium chloride gradient
14N 14 N
14N 14 N
15 N 14 N
15N 14 N
15N 15 N
Results confirm prediction of semiconservative replication.

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