Here, you can find an example of a paper chromatography experiment. Use the two Chromatograms below the answer the questions that follow: a) What are the components of unknown sample (X)? b) Calculate the Rf values for A, B, C in the first chromatogram. d) When using the 20% Acetone in Water, are the substances strongly attracted to the stationary phase or the mobile Phase? Briefly explain your reasoning. e) Which substance does show strongest affinity to the stationary phase in the first chromatogram. Briefly explain your reasoning. wwwni www. 50 80% Acetone in water 20% Acetone in water Finish line 50 45 45 40 40 35 35 30 30 25 25 20 20 15 15 10 Starting line A B C D X A B C D X

Here, you can find an example of a paper chromatography experiment. Use the two Chromatograms below the answer the questions that follow: a) What are the components of unknown sample (X)? b) Calculate the Rf values for A, B, C in the first chromatogram. d) When using the 20% Acetone in Water, are the substances strongly attracted to the stationary phase or the mobile Phase? Briefly explain your reasoning. e) Which substance does show strongest affinity to the stationary phase in the first chromatogram. Briefly explain your reasoning. wwwni www. 50 80% Acetone in water 20% Acetone in water Finish line 50 45 45 40 40 35 35 30 30 25 25 20 20 15 15 10 Starting line A B C D X A B C D X

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Which statement is true? Statement 1: In chromatography, the compound mixture moves along with the mobile phase through the stationary phase and the components get separated depending on the degree of interaction and adhesion Statement 2: Compound A is more soluble in the mobile phase compared to compound B. Thus, compound A would most probably have a higher Rf value.
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Write TRUE if the BOLD word/phrase makes the statement correct. Otherwise, write the correct WORD/PHRASE that will make the statement true. If there are two bold words/phrases in a number, write your answer for EACH of the bold words/phrases. 1.The stationary phase in the paper chromatography experiment is filter paper. 2. When spots are too concentrated, the spots will appear as a streak rather than a spot.
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Next, 2.00 mL of 9.63 mM S was added to 10.00 mL of a solution of unknown concentration of X, and the mixture was diluted to 25.0mL. Chromatographic analysis of this solution produced peak areas of 4822 for X and 4099 for S. What was the concentration of 5 (in mM) of the 25.0mL solution?
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You run the chromatography system with paper as the stationary phase and 70% isopropanol as the mobile phase. You determine the Rf of a polar analyte to be 0.40. If you use 100% isopropanol (a less polar solvent) instead of isopropanol as the mobile phase what would you expect to happen to the Rf value? No value effect on Rf higher lower could be higher or lower
If the endpoints you reached during standardization of your NaOH solution were very precise (relative average deviation if 0.03%) but were all overshot (meaning too much NaOH was added an you passedyour equivalence point), is the reported molar concentration of the acid solution from this experiment greater than , less than or equal to the true concentration? Explain.
Basic Analytical Chemistry
Two group of students wish to analyse caffeine content in tea samples. Group 1 performed the analysis using thin layer chromatography. Group 2 wish to conduct the analysis using analytical instrumentation. Suggest a possible analytical instrument method for Group 2.Briefly explain the procedural steps before instrumentation. List another two applications of this instrument.
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