Herbert Boyer and Stanley Cohen pioneered the technique of DNA cloning allowing genes to be transferred from another biological species easily. Their work also gave rise to the development of different recombinant proteins with therapeutic applications like insulin and growth hormone. The former was cloned using Escherichia coli. coli in 1978. With this breakthrough, the first licensed drug produced using recombinant DNA technology was human insulin, developed by Genentech, licensed and marketed by Eli Lilly in 1982. Scientists were able to identify and isolate the gene fragment or the gene of interest, in this case, the gene that is responsible for producing insulin. Moreover, they were able to isolate the bacterial DNA of E. coli. The plasmid and DNA fragment were cut using a restriction enzyme. This DNA fragment was inserted into the plasmid using a DNA ligase. When the DNA fragment was then placed into the bacterial DNA, it was then introduced to the host cell (E. coli) and was then amplified for the selection of transformed host cells. What is the five steps in Recombinant DNA?
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
Herbert Boyer and Stanley Cohen pioneered the technique of DNA cloning allowing genes to be transferred from another biological species easily. Their work also gave rise to the development of different recombinant proteins with therapeutic applications like insulin and growth hormone. The former was cloned using Escherichia coli. coli in 1978. With this breakthrough, the first licensed drug produced using recombinant DNA
technology was human insulin, developed by Genentech, licensed and marketed by Eli Lilly in 1982. Scientists were able to identify and isolate the gene fragment or the gene of interest, in this case, the gene that is responsible for producing insulin. Moreover, they were able to isolate the bacterial DNA of E. coli. The plasmid and DNA fragment were cut using a restriction enzyme. This DNA fragment was inserted into the plasmid using a DNA ligase. When the DNA fragment was then placed into the bacterial DNA, it was then introduced to the host cell (E. coli) and was then amplified
for the selection of transformed host cells.
What is the five steps in Recombinant DNA?
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