Biotechnology is a constantly developing field with many exciting applications. It focuses on the use of living organisms and their biological processes to make useful products and address practical problems. This includes advancements in clinical diagnostics, epidemiological tracking, forensics, industry, and many other areas. In this simulation, you will have the opportunity to perform molecular techniques commonly used in the field of biotechnology to create medically important compounds such as insulin and human growth hormone.Bacteria can obtain naked DNA from their environment in a process called transformation. This process occurs naturally but can also be used in the genetic engineering field to alter the genetic makeup of bacteria. In this simulation, you transform bacteria with a jellyfish gene whose product is green fluorescent protein. The transformed bacteria now have the genetic information to produce GFP (green fluorescent protein), causing them to glow green under fluorescent light. a) explain in your own words how to prepare sample for transformation b) explain in your own words how to Perform heat shock and bacterial recovery c) explain in your own words how to incoulate plates with positive pGLO and negative pGLO

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Biotechnology is a constantly developing field with many exciting applications. It focuses on the use of living organisms and their biological processes to make useful products and address practical problems. This includes advancements in clinical diagnostics, epidemiological tracking, forensics, industry, and many other areas. In this simulation, you will have the opportunity to perform molecular techniques commonly used in the field of biotechnology to create medically important compounds such as insulin and human growth hormone.Bacteria can obtain naked DNA from their environment in a process called transformation. This process occurs naturally but can also be used in the genetic engineering field to alter the genetic makeup of bacteria. In this simulation, you transform bacteria with a jellyfish gene whose product is green fluorescent protein. The transformed bacteria now have the genetic information to produce GFP (green fluorescent protein), causing them to glow green under fluorescent light.

a) explain in your own words how to prepare sample for transformation

b) explain in your own words how to Perform heat shock and bacterial recovery

c) explain in your own words how to incoulate plates with positive pGLO and negative pGLO

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