Given the spectra below, answer the following questions, both spectra were obtained with 1.0 cm optical path: (I) Which region is being studied (II)G and 6 T-G are molecules or atoms, justify your answer (III) What is λmax of G and 6 GT? (IV) If G has a concentration of 0.25 mM, what is its absorptivity at 250 nm? (V) If G has a concentration of 0.25 mM, what is its absorptivity at 350 nm (VI) If 6 GT has a concentration of 0.4 mM, what is your absorptivity at 250 nm? (VII) If G has a concentration of 0.24 mM, what is your absorptivity at 350 nm? (VIII) One solution contains 0.42 mM G and 0.03 mM 6 GT, what will be the absorbance of this solution in 250 and 350 nm ?

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Given the spectra below, answer the following questions, both spectra were obtained with 1.0 cm optical path:

(I) Which region is being studied

(II)G and 6 T-G are molecules or atoms, justify your answer

(III) What is λmax of G and 6 GT?

(IV) If G has a concentration of 0.25 mM, what is its absorptivity at 250 nm?

(V) If G has a concentration of 0.25 mM, what is its absorptivity at 350 nm

(VI) If 6 GT has a concentration of 0.4 mM, what is your absorptivity at 250 nm?

(VII) If G has a concentration of 0.24 mM, what is your absorptivity at 350 nm?

(VIII) One solution contains 0.42 mM G and 0.03 mM 6 GT, what will be the absorbance of this solution in 250 and 350 nm ?

014
6-TG
012
010
0.08-
0.06
04
0.02
0.00
250
300
350
400
WAVE-LENGTH (nm)
ABSORBANCE
Transcribed Image Text:014 6-TG 012 010 0.08- 0.06 04 0.02 0.00 250 300 350 400 WAVE-LENGTH (nm) ABSORBANCE
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