Give a step by step method and diagram of how reversible terminator sequencing works.

Q: What is one major way in which next generation sequencing differs from dideoxy sequencing (Sanger)?

A: The technique of sequencing DNA involves identifying the nucleic acid sequence, or the arrangement…

Q: Describe the shotgun method for sequencing an entire genome.

A: Genome or genetic material of a cell is the DNA content present in the nucleus. DNA or…

Q: Differentiate between Sanger sequencing and next generation sequencing?

A: The process of determining the nucleic acid sequence is known as DNA sequencing.

Q: Briefly explain how a gene can be isolated through positional cloning.

A: A positional cloning is a technique used in laboratory for locating the position of a gene…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.