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Gene of interest T-DNA vector kan (confers kanamycin resistance) Site where Gene of interest is inserted into T DNA restriction the T DNA of the T-DNA vector. enzyme cuts Recombinant T-DNA vector The recombinant T-DNA vector is transformed into A. tumefaciens. A. tumefaciens Recombinant T-DNA vector Plant cells are exposed to A. tumefaciens. The T DNA is transferred and incorporated into the plant cell chromosome. Carbenicillin and kanamycin are then added to kill A. tumefaciens cells and plant cells that have not taken up T DNA, respectively. Plant cell Inserted T DNA carrying new gene The plant cells are transferred to a medium containing growth hormones to regenerate an entire plant. Plant with cloned gene FIGURE 22.14 The transfer of genes into plants using a T-DNA vector from A. tumefaciens.

Gene of interest T-DNA vector kan (confers kanamycin resistance) Site where Gene of interest is inserted into T DNA restriction the T DNA of the T-DNA vector. enzyme cuts Recombinant T-DNA vector The recombinant T-DNA vector is transformed into A. tumefaciens. A. tumefaciens Recombinant T-DNA vector Plant cells are exposed to A. tumefaciens. The T DNA is transferred and incorporated into the plant cell chromosome. Carbenicillin and kanamycin are then added to kill A. tumefaciens cells and plant cells that have not taken up T DNA, respectively. Plant cell Inserted T DNA carrying new gene The plant cells are transferred to a medium containing growth hormones to regenerate an entire plant. Plant with cloned gene FIGURE 22.14 The transfer of genes into plants using a T-DNA vector from A. tumefaciens.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Which portion of a T-DNA vector is transferred to a plant?

Gene of interest T-DNA vector kan (confers kanamycin resistance) Site where Gene of interest is inserted into T DNA restriction the T DNA of the T-DNA vector. enzyme cuts Recombinant T-DNA vector The recombinant T-DNA vector is transformed into A. tumefaciens. A. tumefaciens Recombinant T-DNA vector Plant cells are exposed to A. tumefaciens. The T DNA is transferred and incorporated into the plant cell chromosome. Carbenicillin and kanamycin are then added to kill A. tumefaciens cells and plant cells that have not taken up T DNA, respectively.
Transcribed Image Text:Gene of interest T-DNA vector kan (confers kanamycin resistance) Site where Gene of interest is inserted into T DNA restriction the T DNA of the T-DNA vector. enzyme cuts Recombinant T-DNA vector The recombinant T-DNA vector is transformed into A. tumefaciens. A. tumefaciens Recombinant T-DNA vector Plant cells are exposed to A. tumefaciens. The T DNA is transferred and incorporated into the plant cell chromosome. Carbenicillin and kanamycin are then added to kill A. tumefaciens cells and plant cells that have not taken up T DNA, respectively.
Plant cell Inserted T DNA carrying new gene The plant cells are transferred to a medium containing growth hormones to regenerate an entire plant. Plant with cloned gene FIGURE 22.14 The transfer of genes into plants using a T-DNA vector from A. tumefaciens.
Transcribed Image Text:Plant cell Inserted T DNA carrying new gene The plant cells are transferred to a medium containing growth hormones to regenerate an entire plant. Plant with cloned gene FIGURE 22.14 The transfer of genes into plants using a T-DNA vector from A. tumefaciens.
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