Gene of interest T-DNA vector kan (confers kanamycin resistance) Site where Gene of interest is inserted into T DNA restriction the T DNA of the T-DNA vector. enzyme cuts Recombinant T-DNA vector The recombinant T-DNA vector is transformed into A. tumefaciens. A. tumefaciens Recombinant T-DNA vector Plant cells are exposed to A. tumefaciens. The T DNA is transferred and incorporated into the plant cell chromosome. Carbenicillin and kanamycin are then added to kill A. tumefaciens cells and plant cells that have not taken up T DNA, respectively. Plant cell Inserted T DNA carrying new gene The plant cells are transferred to a medium containing growth hormones to regenerate an entire plant. Plant with cloned gene FIGURE 22.14 The transfer of genes into plants using a T-DNA vector from A. tumefaciens.
Gene of interest T-DNA vector kan (confers kanamycin resistance) Site where Gene of interest is inserted into T DNA restriction the T DNA of the T-DNA vector. enzyme cuts Recombinant T-DNA vector The recombinant T-DNA vector is transformed into A. tumefaciens. A. tumefaciens Recombinant T-DNA vector Plant cells are exposed to A. tumefaciens. The T DNA is transferred and incorporated into the plant cell chromosome. Carbenicillin and kanamycin are then added to kill A. tumefaciens cells and plant cells that have not taken up T DNA, respectively. Plant cell Inserted T DNA carrying new gene The plant cells are transferred to a medium containing growth hormones to regenerate an entire plant. Plant with cloned gene FIGURE 22.14 The transfer of genes into plants using a T-DNA vector from A. tumefaciens.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question
Which portion of a T-DNA vector is transferred to a plant?

Transcribed Image Text:Gene of interest
T-DNA vector
kan
(confers
kanamycin
resistance)
Site where
Gene of interest is inserted into
T DNA
restriction
the T DNA of the T-DNA vector.
enzyme cuts
Recombinant
T-DNA vector
The recombinant T-DNA vector is
transformed into A. tumefaciens.
A. tumefaciens
Recombinant
T-DNA vector
Plant cells are exposed to A. tumefaciens.
The T DNA is transferred and incorporated
into the plant cell chromosome. Carbenicillin
and kanamycin are then added to kill
A. tumefaciens cells and plant cells that
have not taken up T DNA, respectively.

Transcribed Image Text:Plant
cell
Inserted T DNA carrying new gene
The plant cells are transferred to a medium
containing growth hormones to regenerate
an entire plant.
Plant with cloned gene
FIGURE 22.14 The transfer of genes into plants
using a T-DNA vector from A. tumefaciens.
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