A 1 mL sample of glycogen was calculated to contain 35 µmol (micromole) glucose. To 1 mL of this sample was added 2 mL of 2 M HCL. It was then hydrolysed by boiling the solution for 15 minutes. After boiling the hydrolysate was cooled and made up with H2O to a final volume of exactly 10 mL. The glucose was measured in this solution and found to have a concentration of 570 µg/mL (microgram/milliliter).i) Calculate the mass (mg) of glucose in the 10 mL of hydrolysate. As the 1mL of glycogen sample was made up to a final volume of 10 mL, this mass of glucose was produced by the hydrolysis of the original 1 mL glycogen sample.ii) Calculate the amount (µmol) of glucose produced by the hydrolysis of the glycogen sample.iii) Calculate the purity of the glycogen used in the sample as% Purity = (moles of measured glucose/ moles of calculated glucose in glycogen) *100iv) state your answer in a complete sentence.

Given, Volume of sample of glycogen = 1 mL Moles of glucose = 35 micromole HCl volume = 2mL…

Answered: A 1 mL sample of glycogen was…

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Related questions

Concept explainers

Question

A 1 mL sample of glycogen was calculated to contain 35 µmol (micromole) glucose. To 1 mL of this sample was added 2 mL of 2 M HCL. It was then hydrolysed by boiling the solution for 15 minutes. After boiling the hydrolysate was cooled and made up with H₂O to a final volume of exactly 10 mL. The glucose was measured in this solution and found to have a concentration of 570 µg/mL (microgram/milliliter).

i) Calculate the mass (mg) of glucose in the 10 mL of hydrolysate. As the 1mL of glycogen sample was made up to a final volume of 10 mL, this mass of glucose was produced by the hydrolysis of the original 1 mL glycogen sample.
ii) Calculate the amount (µmol) of glucose produced by the hydrolysis of the glycogen sample.

iii) Calculate the purity of the glycogen used in the sample as

% Purity = (moles of measured glucose/ moles of calculated glucose in glycogen) *100

iv) state your answer in a complete sentence.

Expert Solution

Trending now

This is a popular solution!

Step by step

Solved in 3 steps

SEE SOLUTION Check out a sample Q&A here

Follow-up Questions

Read through expert solutions to related follow-up questions below.

Follow-up Question

For the percent purity section should the 35 be converted into moles of calculated glucose as it is currently in micromoles

Solution

by Bartleby Expert

SEE SOLUTION

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions