Fill compare a few different developing solvents and examine the effect of the polarity of the cluent on the Rf values of spots. Task (you may collaborate with one another person): Prepare three 2cm x Sem TLC plates, each spotted with the most polar and least polar compound of the six samples. Run cone in the same 9:1 hexane: ethyl acetate. Run the second one in 1:1 hexane: ethyl acetate. When one of the TLC plates are done and spots clearly marked, empty the content of one of the beakers and try the third cluent: hexane-only. Q7) Arrange the TLC plates developed from these three eluents in the order of eluent polarity (least polar to most polar). Refer to the "Common chromatography solvents" chart. Sketch the three TLC plates in this order. Least polar solvent medium polar solvent Most polar solvent Q8) What is the trend observed here? How does the polarity of developing solvent correlate to the Rf values? (No Rf calculation necessary: higher Rf means the spot traveled more.) Q9) Circle the correct statement that fits the observation made above. (a) The most polar solvent had stronger interaction with the most polar spot, and made it travel more than the least polar spot. (b) The most polar solvent still moved the least polar spot higher, because silica surface drags the more polar spot more strongly.

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UV Visible Spectroscopy

Spectroscopy refers to the absorption or emission of light or other radiations by matter. When a substance or chemical absorbs light or radiation, they reach an excited state followed by de-excitation or emission of the light or radiation forming distinct spectra. UV-visible spectroscopy or  ultraviolet-visible refers to the absorption and emission in the ultraviolet and visible regions of the electromagnetic spectrum.