Explain in lengthy the principle of electrophoresis in nucleic acid isolation. Explain in detail the principle of DNA Isolation Discuss comprehensively why there is a need to remove impurities in DNA samples?

Q: Contrast the processes used by bacterial cells and eukaryotic cells to package their DNA.

A: Asked : Processes used by bacterial cells and eukaryotic cells to package their DNA

Q: Enzymes of bacterial origin used in a wide variety of techniques are: ligases restriction…

A: DNA polymerase

Q: Explain why a quantitative PCR analysis cannot determine the size of the initial template sequence…

A: The combination of the probe-based and intercalating dye-based methods brings the potential…

Q: Describe the components vital for DNA sequencing with the Sanger method

A: DNA sequencing: The process of identifying the correct order of the four nitrogen bases (adenine,…

Q: Protocols in isolating DNA often involve the use of two kinds of ethanol, 100% ethanol and  70%…

A: 70% ethanol is used in washing DNA furing DNA extraction. 100% ethanol is used in the final step. In…

Q: Describe a method of labeling a DNA probe. Explain how this probe maybe detected after DNA…

A: The probe is basically a single stranded sequence of DNA or RNA which is used commonly for the…

Q: Coomassie dye is the equivalent of what component used in analysis of DNA by agarose gel…

A: Western blot helps to identify specific proteins from a mixture of proteins and this step follows…

Q: What are possible mistakes that can cause the DNA to be contaminated during isolation, purification…

A: Various techniques are used to extract different types of DNA. Cells are broken open using a…

Q: Explain the Doyle and Doyle (1990) method of extraction. Identify which step removes specific…

A: Introduction: Since Friedrich Miescher's original DNA extraction in 1869, scientists have made…

Q: Describe the methods for visualizing DNA molecules after separation.

A: Electrophoresis is a technique to be analyzed at the level of its constituent molecules. In this,…

Q: Based on the following image: A) Identify the largest DNA fragment in sample 5. Explain your…

A: In the given question, an agarose gel is shown which is having bands of DNA in different lanes. DNA…

Q: Explain the use of a probe in screening a DNA library

A: Introduction: Probes are the small DNA or RNA strands that are used to label DNA. The labeling of…

Q: explain how bacteria can be manipulated to contain a foreign DNA sequence or gene, how the…

A: Plasmid or vector transformation is the process by which exogenous DNA is transferred into the host…

Q: Summarize the process by which gel electrophoresisseparates DNA fragments.

A: Gel electrophoresis is used in the separation of many macromolecules like DNA, RNA, and proteins.…

Q: Which RE can be used to distinguish bacterial strain 1 from strain 2 based on a sample DNA fragment…

A: Restriction enzymes are a type of nuclease that cuts DNA at a specific sequence of nucleotides. They…

Q: Recombinant DNA techniques that require gel electrophoresis

A: Following are the applications of gel electrophoresis in DNA recombinant technology.

Q: In ion torrent DNA sequencing, covalent bond formation is detected by an ion sensor sensitive to…

A: It's a DNA sequencing method

Q: Briefly explain how the polymerase chain reaction is used to amplify a specific DNA sequence. What…

A: PCR which stands for polymerization chain reaction is a rapid and extremely sensitive analytical…

Q: If we extract DNA of a pool of cells, will that impact the DNA sequencing? In other words, having…

A: Since we only answer one question at a time according to our honor code, therefore we’ll answer…

Q: Explain how DNA replication is used to make base sequencing possible with Sanger's method and the…

A: DNA sequencing is done to find out the order of nucleotides along a DNA strand.The chemical building…

Q: Explain how DNA replication is used to make base sequencing possible with Sanger’s method and the…

A: Sequencing is the determination of the order of nucleotides bases in a DNA (deoxyribonucleic acid)…

Q: Diagram the steps for the production of recombinant DNA. Label and Identify the steps involved and…

A: In the image provided above, the bacterial plasmid is composed of restriction sites such as EcoRI…

Q: describe the two main parts of a plasmid DNA extraction and purification procedure including the…

A: Plasmid DNA extraction

Q: Explain why a positive control and negative control are included in PCR experiments. ​Explain the…

A: PCR is a laboratory technique that is used for making large number of copies of a target DNA…

Q: Provide other techniques which can be used to characterize DNA extracts. Provide advantages and…

A: DNA extracts characterization is done by many methods. It is done to check purity, stability,…

Q: Define the terms DNA profiling and DNA sequencing.

A: DNA profiling and DNA fingerprinting are two molecular approaches for identifying individuals based…

Q: Briefly describe what happens during each of the phases of PCR (denaturation, annealing, and…

A: The “polymerase chain reaction” (PCR) is a technique in which millions of copies of the target DNA…

Q: using the process flow of PCR describe the principle associated with the PCR technique.

A: Introduction :  Polymerase Chain Reaction or PCR is a widely employed technique in molecular biology…

Q: The purpose of using soap in the DNA extraction activity using frozen strawberries is to

A: A gene is an area of DNA molecule or polymer of amino acid that encodes function. A chromosome…

Q: Use this information to match the list of probes to the 3 samples below: You have isolated several…

A: Southern blotting and Northern blotting are used to isolate and identify nucleic acid present in the…

Q: Elaborate on the basic principle you would adopt to isolate an amplified DNA fragment using agarose…

A: Electrophoresis is a biochemical technique that separates molecules with the help of an electric…