Explain how PCR is used to detect the presence of pathogens in patient samples. Look up an example of where this technique is used routinely in diagnosis.
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Explain how PCR is used to detect the presence of pathogens in patient samples. Look up an example
of where this technique is used routinely in diagnosis.
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- Explain how PCR/OLA (polymerase chain reaction/oligonucleotide ligation assay) can be used in the diagnosis of sickle cell disorder . Would you recommend this method for routine diagnosis of sickle cell disorder? ExplainDescribe what is produced by a PCR assay, how it is detected, and explain how it can determine whether a specific pathogen is present in a sample.Explain how PCR can be used to detect infectious agents in diagnoses of diseases.
- Discuss the uses of PCR and RT-PCR in human identity (e.g. paternity, forensics or criminal cases) as well as in viral or bacterial DNA/RNA analysis (i.e. Identification of SARS-COV-2 RNA coronavirus using RT-PCR).Describe the applications of PCR in identification techniques.ecombinant DNA technology has provided a broad range of tools to help physicians in the diagnosis of diseases. Probes are now used for identification of what infectious agents ?
- Briefly explain Current Infection Marker (CIM) test, Campylobacter-Like Organism (CLO) test, and the multiplex polymerase chain reaction test (PCR). Which of the tests had the highest accuracy and what methodology and techniques is used for Multiplex PCR test.Describe how would you determine the following using a real-time PCR instrument. A) The quantity and quality of a DNA sample. B) Whether an individual had cystic fibrosis from a genomic DNA sample. C) If several bacterial pathogens were present in DNA extracted from a food sample.Explain the relevance of polymerase chain reaction (PCR) in clinical practice.