Experiment: Lipids Qualitative Tests Test Performed: Translucent Test Procedure 1. Fold your filter paper into 3 equal parts. 2. Using a dropper, drop the olive oil on one part, then drop the alcohol on the second part, and drop the ether on the third part of the filter paper. 3. After a few minutes, note the drop made on the filter paper by holding it up to the light. Results and observation: Upon holding the filter paper in the light, only the olive oil has a translucent spot. The alcohol was absorbed by the filter paper making the spot invisible while the ether has a slightly visible mark but was not translucent. Questions: 1. What is the equation involved? 2. What is the principle behind the result?
Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
Experiment: Lipids Qualitative Tests
Test Performed: Translucent Test
Procedure
1. Fold your filter paper into 3 equal parts.
2. Using a dropper, drop the olive oil on one part, then drop the alcohol on the second part, and drop
the ether on the third part of the filter paper.
3. After a few minutes, note the drop made on the filter paper by holding it up to the light.
Results and observation:
Upon holding the filter paper in the light, only the olive oil has a translucent spot. The alcohol was absorbed by the filter paper making the spot invisible while the ether has a slightly visible mark but was not translucent.
Questions:
1. What is the equation involved?
2. What is the principle behind the result?
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