Experiment: Transformation of the bacterium Streptococcus pneumoniae. Based on the study, four mutations were examined. They are penicillin resistance (P), Streptomycin resistance (S), mannitol usage ability (M), sulfanilamide resistance (F). DNA was extracted from the strains of bacteria with different combinations of mutations and the DNA was used to transform wild type bacterial cells (P+F+M+S+) The results of the transformation experiments are: Donor DNA Recipient Percentage of all cells Transformants DNA MSF M S F* M*SF 4.0 M S F. 4.0 MS F 2.6 MSF 0.41 MSF 0.22 MS F 0.0058 MSF 0.0071 Predict the order of M. S and F genes on the bacterial chromosome

Human Anatomy & Physiology (11th Edition)
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Experiment: Transformation of the bacterium Streptococcus pneumoniae. Based on the study, four
mutations were examined. They are penicillin resistance (P), Streptomycin resistance (S), mannitol
usage ability (M), sulfanilamide resistance (F). DNA was extracted from the strains of bacteria with
different combinations of mutations and the DNA was used to transform wild type bacterial cells
(P+F+M+S+) The results of the transformation experiments are:
Transformants Percentage of all
cells
Donor
Recipient
DNA
DNA
MSF
M* S' F*
MSF
4.0
MSF
4.0
MS F
2.6
MSF
0.41
MSF
0.22
MS F
0.0058
MSF
0.0071
Predict the order of M. S and F genes on the bacterial chromosome
Transcribed Image Text:Experiment: Transformation of the bacterium Streptococcus pneumoniae. Based on the study, four mutations were examined. They are penicillin resistance (P), Streptomycin resistance (S), mannitol usage ability (M), sulfanilamide resistance (F). DNA was extracted from the strains of bacteria with different combinations of mutations and the DNA was used to transform wild type bacterial cells (P+F+M+S+) The results of the transformation experiments are: Transformants Percentage of all cells Donor Recipient DNA DNA MSF M* S' F* MSF 4.0 MSF 4.0 MS F 2.6 MSF 0.41 MSF 0.22 MS F 0.0058 MSF 0.0071 Predict the order of M. S and F genes on the bacterial chromosome
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