Expansion microscopy technique beats the 0.2 um resolution limit of optical microscopy by improving the numerical aperture (NA) of the objective lenses of a microscope. A. True B. False
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- Between wet mount and hanging drop slide preparations, which is more resistant to evaporation? Which works best with phase-contrast microscopy?For what purpose would you adjust each of the following microscopecomponents during a microscopy exercise?a. Iris diaphragm:b. Coarse-adjustment knob:c. Fine-adjustment knob:d. Condenser:e. Mechanical stage control:a) Describe the technique Of Atomic Force Microscopy under the following headings: (i) Basic principle of AFM and overview of operations (ii) Modes of A FM
- a. How was the specimen prepared for the microscopy technique applied? (for e.g. stained with H&E stain, Gram stain, unstained) b. What is the microscopy technique and magnification used to obtain this image? c. What is the basic principle of image formation using this microscopy technique? d. What can be observed and concluded from the image of the specimen? e. Are there any potential aberrations present in this specimen image? Describe these and how they may affect interpretation of the result.1. How will the following affect resolution during microscopy?i. Closing or opening the diaphragmii. Raising or lowering the condenserii. Increasing or reducing the light intensity 2. During the calibration of an ocular micrometer, two (2) stage micrometer divisions were observed to line up with ten (10). If the graduation interval on the stage micrometer is 0.01mm, calculate: i. The calibrated value of the ocular micrometer in um.ii. The size of Pseudomonas aeruginosa which has occupied 5 ocular divisions. 3. What are the methods of staining?What is the diameter of the field of view (DFV) of a 1000x objective lens if the DFV of a 400x objective lens is 500 μ? Express your answer in mm.
- B. Write the function/s of each part of the microscope listed below. a. Eyepiece b. Draw tube c. Hemispheric prism housing d. Dust shield e. Revolving Nose Piece f. Objectives: Scanner LPO HPO ΟΙΟ g. Arm h. Coarse Adjustment knob i. Fine adjustment knob j. Slide holder & clip k. Rear knob I. Front knob m. Central aperture n. Condenser o. Iris diaphragm p. Mechanical Stage q. Mirror r. Mirror rack s. Stage adjustment knob t. BaseDifferentiate between the limit of resolution of the typical light microscope and that of the unaided human eyes the relationship between the depth of field and magnification is that When magnification increases, the depth of field decreases. Name two procedures (steps) you should do to achieve the maximum resolution when using the oil immersion lens State the relationship between the working distance of an objective lens and its magnification power “The coarse focus knob can be used to adjust the focus when using any of the objective lenses”. Is this statement true or false? If it is false, please correct it. When using the oil immersion lens but you cannot focus, what should you should not do? And what you should do? What does this statement mean: “Your microscope is parfocal”? What is the significance of condenser lens? Multiple choice question: The most useful adjustment for increasing image contrast in low power magnification is _______ Closing down the diaphragm Closing one…Explain the basic differences between fresh and fixed preparationsfor microscopy and how they are used.
- Complete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. LPO НРО OIO Focal length (mm) Working distance (mm) Linear magnification (X) Numerical aperture (N.A..) Features (color of the band, diameter of the opening of the lens, etc.)Does image brightness increase or decrease when closing the diaphragm during microscopy.Compare and contrast the operation of optical microscopy and TEM in terms of Contrast (explain all different types for each microscopy) Note: Wherever possible, explain what factors affect or control each features.