Erwin Chargaff conducted several studies on the base composition of DNA. The data in Table 1 shows the molar properties of bases in the nucleic acid from six different living organisms. Nucleic acid Yeast Paracentrotus lividus Herring Rat Human Species Z (i) (ii) (iii) Adenine 31.3 32.8 27.8 28.6 30.7 30.9 Table 1 Percentage (%) Thymine 32.9 32.1 27.5 28.4 31.2 29.4 Guanine 18.7 17.7 22.2 21.4 19.3 ? Cytosine 17.1 18.4 22.6 21.5 18.8 ? A+G G+C 1.79 1.85 1.23 1.33 1.62 ? What are the percentage of Guanine and Cytosine in Species Z? Which organism has the higher melting point for its DNA structure compared to Species 7.? Explain why DNA in the presence of salt will have a higher melting temperature (Tm).
Nucleotides
It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic Acids
Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.
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