enzyme has a molecular weight 5 X 10'daltons, a KM value and a k₂ value of k₂=10* molecules ATP/min molecule enzyme at 37 C. The reaction catalyzed is the following ATP → ADP + P This can also be represented as: E + S K₁ K₁ E-S K₂ E + P Here S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics are first order: E = Ege-kat Here Eo is the initial enzyme concentration and ka=0.1 min¹. In an experiment with a partially pure enzyme preparation, 10 µg of total crude protein (containing enzyme) is added to a 1 mL reaction mixture containing 0.02 M ATP and incubated at 37C. After 12 hours, the reaction ends (i.e., t→∞) and the inorganic phosphate (P) concentration is found to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was the enzyme? Hint: since [S]>>>Km, the reaction rate can be represented by: d (P) dt = K₂ [E]
enzyme has a molecular weight 5 X 10'daltons, a KM value and a k₂ value of k₂=10* molecules ATP/min molecule enzyme at 37 C. The reaction catalyzed is the following ATP → ADP + P This can also be represented as: E + S K₁ K₁ E-S K₂ E + P Here S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics are first order: E = Ege-kat Here Eo is the initial enzyme concentration and ka=0.1 min¹. In an experiment with a partially pure enzyme preparation, 10 µg of total crude protein (containing enzyme) is added to a 1 mL reaction mixture containing 0.02 M ATP and incubated at 37C. After 12 hours, the reaction ends (i.e., t→∞) and the inorganic phosphate (P) concentration is found to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was the enzyme? Hint: since [S]>>>Km, the reaction rate can be represented by: d (P) dt = K₂ [E]
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
Related questions
Question
![An enzyme ATPase has a molecular weight of 5 x 104 daltons, a KM value of 104 M and a
k2 value of k₂=104 molecules ATP/min molecule enzyme at 37 C. The reaction catalyzed
is the following
ATP → ADP + P
This can also be represented as:
E + S
k₁
k₁
E-S
K₂
E + P
Here S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation
kinetics are first order:
E = Ege-kat
Here Eo is the initial enzyme concentration and ka=0.1 min¹. In an experiment with a
partially pure enzyme preparation, 10 µg of total crude protein (containing enzyme) is
added to a 1 mL reaction mixture containing 0.02 M ATP and incubated at 37C. After 12
hours, the reaction ends (i.e., t→∞) and the inorganic phosphate (P) concentration is found
to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was
the enzyme?
Hint: since [S]>>>Km, the reaction rate can be represented by:
d(P)
dt
= K₂ [E]](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fe80ef817-aa8b-4047-ac27-a3f534c77dd1%2Fa96b63eb-212b-40a4-9320-8601c68cc73b%2F6f5e54l_processed.png&w=3840&q=75)
Transcribed Image Text:An enzyme ATPase has a molecular weight of 5 x 104 daltons, a KM value of 104 M and a
k2 value of k₂=104 molecules ATP/min molecule enzyme at 37 C. The reaction catalyzed
is the following
ATP → ADP + P
This can also be represented as:
E + S
k₁
k₁
E-S
K₂
E + P
Here S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation
kinetics are first order:
E = Ege-kat
Here Eo is the initial enzyme concentration and ka=0.1 min¹. In an experiment with a
partially pure enzyme preparation, 10 µg of total crude protein (containing enzyme) is
added to a 1 mL reaction mixture containing 0.02 M ATP and incubated at 37C. After 12
hours, the reaction ends (i.e., t→∞) and the inorganic phosphate (P) concentration is found
to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was
the enzyme?
Hint: since [S]>>>Km, the reaction rate can be represented by:
d(P)
dt
= K₂ [E]
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