Enolase has a glutamic acid residue in its active site. Describe the experiment that was done that provided evidence for the proposed function of the glutamic acid residue in catalysis by enolase

Q: Pyridoxal phosphate acts as an electron sink, stabilizing carbanionic intermediates. What other…

A: Pyridoxal phosphate is necessary for a variety of processes in human metabolism, most notably in the…

Q: What are the three reactions catalyzed by PLP? Just name them.

A: Pyridoxal phosphate (PLP) refers to a coenzyme present in a variety of reactions and it is the…

Q: You have discovered a new enzyme that has a nearly identical active site to chymotrypsin. This new…

A: As given peptide is Asn-Phe-lys, and discovered enzyme cleaves at C terminal of non ionizable polar…

Q: , non-ionizable R groups.

A: Introduction  Beginning with the first tetrahedral middle of the road, draw the instrument of…

Q: Given the following enzyme-catalyzed reaction, identify the class and subclass of the enzyme…

A: Amino acids are biomolecules that have an amino group, a carboxyl group and a side group that is…

Q: Select the following enzymes that utilize a mechanism involving covalent catalysis. Triose phosphate…

A: In covalent catalysis, the enzymes temporarily forms a covalent bond with the substrate, forming an…

Q: Michaelis and Menten used the simplified scheme for an enzyme catalyzed reaction: keat k₁ E+S=ES+E+P…

A: Michalis Menten enzyme kinetics is based on the assumption the rapid equilibrium approach i.e.…

Q: What is a word equation for the enzyme catalys phenoloxidase?

A: enzymes are bio catalyst which lower the energy of activation and increase the speed of the reaction…

Q: How can you recognize an enzyme that does not display Michaelis–Menten kinetics?

A: Enzymes are proteins that catalysis a biochemical reaction. Enzymes are restored after the reaction.…

Q: Explain why several biochemical pathways start by putting a coenzyme A onto the molecule that…

A: For the functioning of an enzyme, a non-protein compound is usually needed. They loosely bind to…

Q: why is biocatalysis over inorganic catalysts in enzyme catalysis favored?

A: Those substances that increase the pace of the chemical reaction without undergoing any change…

Q: Radioactive uracil can be used to label all of the pyrimidine residues in DNA. Using either names or…

A: Deoxyribonucleic acid is a molecule composed of two polynucleotide chains that coil around each…

Q: Comment on Figure 2a and Figure 2b regarding stabilization of the transition state and…

A: From the given energy diagrams; It is seen that the enzyme (E) and the substrate (S) at the start of…

Q: Chymptrysin preferentially binds thetransition state. Explain what is meant by this.

A: Chymotrypsin is defined as a “hydrolytic enzyme”. It is a member of the super-family, belonging to…

Q: Which of the following is true under the following conditions: an enzyme displaying Michaelis-Menten…

A: Enzymes are protein molecules that increase the rate of reaction by million times. Enzymes do not…

Q: Briefly describe the role of nucleophilic catalysis in the mechanism of the chymotrypsin reaction.

A: Chymotrypsin is the enzyme synthesized in the pancreas in the form of the inactive state known as…

Q: The compound triclosan inhibits bacterial enoyl-ACP reductase. Explain why triclosan is useful as a…

A: Triclosan is an antibacterial and antifungal agent which is found in some consumer products, such as…

Q: With reference to your experimental protocol, why does adding the reaction mix to the “stop” tubes…

A: Alkaline phosphatase (ALP) is an enzyme that catalyzes the hydrolysis of phosphate esters in an…

Q: Identify each reaction catalyzed by (a) a nucdeotidase; (b) a phosphorylase; (c) a…

A: De novo nucleotides are synthesized by the Salvage pathway. The pathway occurs in Kupffer cells,…

Q: Match the following catalytic strategies with their example. Place a Letter on the picture. There…

A: Enzymes are protein molecules that increase the rate of reactions by decreasing the activation…

Q: Are enzyme-catalyzed reactions examples of homogeneous or heterogeneous catalysis?

A: Homogenous catalysis refers to a catalytic system in which the catalysts and the substances are in…

Q: (Part B) Coenzyme-dependent enzymes can catalyze the general transformations shown below. What would…

A: Coenzymes are the cofactors or the organic molecule which helps the enzymes in catalyzing various…

Q: Several factors contribute to enzyme catalysis. What arethey? Briefly explain the effect of each.

A: Enzymes are proteins that act as biocatalysts in speeding up biochemical reactions without actually…

Q: An enzyme with an unknown reaction mechanism has been isolated and is being investigated in the lab.…

A: General acid/base: proton transfer occurs between enzyme and substrate. Note that water is not a…

Q: When enzyme solutions are heated, there is a progessive loss of catalytic activty over time due to…

A: Enzyme is defined as a biological catalyst that speeds up the rate of chemical reaction. All…

Q: Lysozyme catalyzes a "bi-bi" reaction. List, in order, the reactants that bind and the products that…

A: Enzymes are biological molecules (typically protein) that significantly speed up the rate of…

Q: Construct the mechanism of a serine-protease with a catalytic triad.

A: A serine protease is an enzyme that cleaves peptide bonds in proteins and peptides. It possesses a…

Q: H -ОН H-OH H— CH₂OPO3²- CH₂ HO-LH H-OH H-OH ÖH2OPO32- 2-О3РО. со (D) 2-3РОН2 (A) 2-O3PO O_CO CH₂…

A: There are three Aromatic amino acids: Phe, Tyr and Trp. All of them are synthesized from common…

Q: Which strategies listed does this enzyme use to facilitate cleavage of α-galactoside? Choice 1 of…

A: Alpha-galactosidase enzymes are homodimeric glycoprotein which catalyzes the cleavage of terminal…

Q: Briefly explain the role of the oxyanion hole for the catalysis of chymotrypsin.

A: Chymotrypsin is a seine protease. Serine protease is a class of enzyme in which catalytic mechanism…

Q: What are cofactors and coenzymes in an enzymatic reaction? Is tetrahydro- folate considered a…

A: Enzymes require cofactor and coenzyme for their activity.

Q: What role do low-barrier hydrogen bonds play in serine protease catalysis?

A: A low barrier hydrogen bond is a special type of hydrogen bond which can occur when the pKa of the…

Q: How does the Michaelis-Menten equation explain why the rate of an enzyme-catalyzed reaction reaches…

A: This scheme for the most part clarifies the onserved kinetics since it shown the rate being…

Q: Match the following catalytic strategies with their example. Place a Letter on the picture. There…

A: Enzymes are biological catalysts that increase the rate of a chemical reaction. They are exhibit…

Q: Which of the following statements is correct for the reaction catalyzed by chymotrypsin? ○ The…

A: For the reaction catalyzed by chymotrypsin, the correct statement is:The substrate carbon atom…

Q: Considering PNPP → PNP reaction, would you expect to see more intense or pale color for the reaction…

A: para-Nitrophenylphosphate (pNPP) is a chromogenic substrate for phosphatases used in…

Q: Explain how the following changes affect the rate of an enzyme-catalyzed reaction in the presence of…

A: In uncompetitive inhibition, the enzyme inhibitor binds to the enzyme-substrate complex. This type…