Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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![Given the assumption used to derive the Michaelis-Menten equation for enzyme kinetics that the back
reaction from P + E back to ES does not occur, why is it important in measuring experimental data for
Michaelis-Menten analysis to determine the initial rate of the reaction just after adding substrate to the
sample being measured?
O Because faster rates can be measured more accurately than slower rates later in the process.
Because the Michaelis-Menten equation also contains the position of the equilibrium of the reaction.
● By using this approach, the amount of P at the start of the experiment is extremely low, causing the back reaction to be
essentially absent.
Because in this way the [ES] concentration has not yet reached steady state.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F0dda238e-660e-4563-b5ca-f2ea67268dd8%2Ff60ffbf5-17c8-4c1d-8b72-08ae67d43f77%2Fmm1nbz_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Given the assumption used to derive the Michaelis-Menten equation for enzyme kinetics that the back
reaction from P + E back to ES does not occur, why is it important in measuring experimental data for
Michaelis-Menten analysis to determine the initial rate of the reaction just after adding substrate to the
sample being measured?
O Because faster rates can be measured more accurately than slower rates later in the process.
Because the Michaelis-Menten equation also contains the position of the equilibrium of the reaction.
● By using this approach, the amount of P at the start of the experiment is extremely low, causing the back reaction to be
essentially absent.
Because in this way the [ES] concentration has not yet reached steady state.
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