Ellis Engelsberg and his colleagues examined the regulation of genes taking part in the metabolism of arabinose, a sugar (E. Engelsberg et al. 1965. Journal of Bacteriology 90:946-957). Four structural genes encode enzymes that help metabolize arabinose (genes A, B, D, and E). An additional sequence C is linked to genes A, B, and D. These are in the order D- A- B- C. Gene E is distant from the other genes. Engelsberg and his colleagues isolated mutations at the C sequence that affected the expression of structural genes A, B, D, and E. In one set of experiments, they created various genotypes at A and C and determined whether arabinose isomerase (the enzyme encoded by gene A) was produced in the presence or absence of arabinose (the substrate of arabinose isomerase) by cells with these genotypes. Results from this experiment are shown in the following table, where a plus sign (+) indicates that the arabinose isomerase was synthesized and a minus sign (-) indicates that the enzyme was not synthesized. Arabinose Arabir Genotype absent prese 1. C* A* 2. C A* 3. C A*/C* 4. CA/C A*

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Chapter1: The Human Body: An Orientation
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Do these experiments suggest that the arabinose operon is negatively or positively controlled? Explain your reasoning.

Ellis Engelsberg and his colleagues examined the regulation of genes
taking part in the metabolism of arabinose, a sugar (E. Engelsberg et al.
1965. Journal of Bacteriology 90:946-957). Four structural genes
encode enzymes that help metabolize arabinose (genes A, B, D, and E).
An additional sequence C is linked to genes A, B, and D. These are in
the order D- A- B- C. Gene E is distant from the other genes. Engelsberg
and his colleagues isolated mutations at the C sequence that affected the
expression of structural genes A, B, D, and E. In one set of experiments,
they created various genotypes at A and C and determined whether
arabinose isomerase (the enzyme encoded by gene A) was produced in
the presence or absence of arabinose (the substrate of arabinose
isomerase) by cells with these genotypes. Results from this experiment
are shown in the following table, where a plus sign (+) indicates that the
arabinose isomerase was synthesized and a minus sign (-) indicates that
the enzyme was not synthesized.
Arabinose
Arabir
Genotype
absent
prese
1. C* A*
2. C A*
3. C A*/C*
4. CA/C
A*
Transcribed Image Text:Ellis Engelsberg and his colleagues examined the regulation of genes taking part in the metabolism of arabinose, a sugar (E. Engelsberg et al. 1965. Journal of Bacteriology 90:946-957). Four structural genes encode enzymes that help metabolize arabinose (genes A, B, D, and E). An additional sequence C is linked to genes A, B, and D. These are in the order D- A- B- C. Gene E is distant from the other genes. Engelsberg and his colleagues isolated mutations at the C sequence that affected the expression of structural genes A, B, D, and E. In one set of experiments, they created various genotypes at A and C and determined whether arabinose isomerase (the enzyme encoded by gene A) was produced in the presence or absence of arabinose (the substrate of arabinose isomerase) by cells with these genotypes. Results from this experiment are shown in the following table, where a plus sign (+) indicates that the arabinose isomerase was synthesized and a minus sign (-) indicates that the enzyme was not synthesized. Arabinose Arabir Genotype absent prese 1. C* A* 2. C A* 3. C A*/C* 4. CA/C A*
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