Ellis Engelsberg and his colleagues examined the regulation of genes taking part in the metabolism of arabinose, a sugar (E. Engelsberg et al. 1965. Journal of Bacteriology 90:946-957). Four structural genes encode enzymes that help metabolize arabinose (genes A, B, D, and E). An additional sequence C is linked to genes A, B, and D. These are in the order D- A- B- C. Gene E is distant from the other genes. Engelsberg and his colleagues isolated mutations at the C sequence that affected the expression of structural genes A, B, D, and E. In one set of experiments, they created various genotypes at A and C and determined whether arabinose isomerase (the enzyme encoded by gene A) was produced in the presence or absence of arabinose (the substrate of arabinose isomerase) by cells with these genotypes. Results from this experiment are shown in the following table, where a plus sign (+) indicates that the arabinose isomerase was synthesized and a minus sign (-) indicates that the enzyme was not synthesized. Arabinose Arabir Genotype absent prese 1. C* A* 2. C A* 3. C A*/C* 4. CA/C A*
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
Do these experiments suggest that the arabinose operon is negatively or positively controlled? Explain your reasoning.

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