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EFFECT OF TEMPERATURE ON ENZYME ACTIVITY Results: Tub Temperature Benedict's test Iodine test Ice (0º) Room Temperature 3 40°C 4 60°C 5 Boil (100)

EFFECT OF TEMPERATURE ON ENZYME ACTIVITY Results: Tub Temperature Benedict's test Iodine test Ice (0º) Room Temperature 3 40°C 4 60°C 5 Boil (100)

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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EFFECT OF TEMPERATURE ON ENZYME ACTIVITY Results: Tub Temperature Benedict's test Iodine test e 1 Ice (0º) 2 Room Temperature 3 40°C 4 60°C 5 Boil (100°)
Transcribed Image Text:EFFECT OF TEMPERATURE ON ENZYME ACTIVITY Results: Tub Temperature Benedict's test Iodine test e 1 Ice (0º) 2 Room Temperature 3 40°C 4 60°C 5 Boil (100°)
EFFECT OF ENZYME CONCENTRATION ON ENZYME ACTIVITY Procedure: 1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva to each tube. Mix thoroughly. 2. Place the first tube in ice water, the 2nd tube leave at room temperature, the 3rd tube in 400C, the 4th tube at 60oCwater bath and the 5th tube boil for 2 minutes.. 3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4th tube allows to stand for 30 minutes after heating for 2 minutes. 4. Test the contents of each tube with iodine and benedict's tests. a. lodine Test – to half of the content of each tube, add 1 drop of iodine in KI and note the color produced. Compare the intensity of color in each of the 6 tubes. b. Benedict's – to 1 ml benedict's reagent, - add 5 drops of the other half of each tube and heat in a boiling water bath for 3 minutes. Note color of precipitate
Transcribed Image Text:EFFECT OF ENZYME CONCENTRATION ON ENZYME ACTIVITY Procedure: 1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva to each tube. Mix thoroughly. 2. Place the first tube in ice water, the 2nd tube leave at room temperature, the 3rd tube in 400C, the 4th tube at 60oCwater bath and the 5th tube boil for 2 minutes.. 3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4th tube allows to stand for 30 minutes after heating for 2 minutes. 4. Test the contents of each tube with iodine and benedict's tests. a. lodine Test – to half of the content of each tube, add 1 drop of iodine in KI and note the color produced. Compare the intensity of color in each of the 6 tubes. b. Benedict's – to 1 ml benedict's reagent, - add 5 drops of the other half of each tube and heat in a boiling water bath for 3 minutes. Note color of precipitate
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