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Eco01091 20/4 Plai 40 PotAPI 179 Aatl 2613 Niel 183 Sspl 210 Ehel, SspDI 235 Pdmi 220 Begl 2215 Scal 217 Tsol 2108 2486 MCS BSAXI 659 PUC18/19 2686 bp NmeAlli 1822. Af, Pscl 806 Gsul 1/84- Ctri01 17/9 '826 466 Eco311 1/66 Eam1051 169-1. rep (PMB1) Pspi 1110 ail 1217 Figure 1 (https://www.fishersel.com/shop/products/thermo-scientific-puc18-puc19-dna-50-g/fersd0051) Figure I above is showing the plasmid map of PUC18, Discuss how screening is carried out if this plasmid is used for cloning. Explain the THREE (3) important regions of this plasmid that enable it to work efficiently as a cloning vector? b) State the differences between the plasmid in Figure I with PBR322 in terms of their sereening procedures. c) Discuss TWO (2) methods of transformation of competent bacterial cells with the plasmid in Figure 1. d) bla (Ap)

Eco01091 20/4 Plai 40 PotAPI 179 Aatl 2613 Niel 183 Sspl 210 Ehel, SspDI 235 Pdmi 220 Begl 2215 Scal 217 Tsol 2108 2486 MCS BSAXI 659 PUC18/19 2686 bp NmeAlli 1822. Af, Pscl 806 Gsul 1/84- Ctri01 17/9 '826 466 Eco311 1/66 Eam1051 169-1. rep (PMB1) Pspi 1110 ail 1217 Figure 1 (https://www.fishersel.com/shop/products/thermo-scientific-puc18-puc19-dna-50-g/fersd0051) Figure I above is showing the plasmid map of PUC18, Discuss how screening is carried out if this plasmid is used for cloning. Explain the THREE (3) important regions of this plasmid that enable it to work efficiently as a cloning vector? b) State the differences between the plasmid in Figure I with PBR322 in terms of their sereening procedures. c) Discuss TWO (2) methods of transformation of competent bacterial cells with the plasmid in Figure 1. d) bla (Ap)

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Eco01091 20/4 Plai 48 PstAPI 179 Aath 2613 Liet 183 Sspl 2501 Ehel, SspDI 235 Pdmi 2234 Bogl 2215 Scal 2177 Tsol 2108 2456 MCS BsaX 659 pUC18/19 2686 bp NmeAlli 1822 Afl, Pscl 806 Gsul 1/84 Ctri0l 17/19, Eco311 1/66 Eam1105! I691 466 rep (PMB1) Cail 1217 Psp-i 1110 Figure 1 (https://www.fishersci.com/shop/products/thermo-scientific-puc18-pue19-dna-50-g/fersd0051) Figure I above is showing the plasmid map of PUC18. Discuss how screening is carried out if this plasmid is used for cloning. Explain the THREF. (3) important regions of this plasmid that enable it to work efficiently as a cloning vector? b) State the differences between the plasmid in Figure i with PBR322 in terms of their sercening procedures. c) Discuss TWO (2) methods of transformation of competent bacterial cells with the plasmid in Figure 1. d) bla (Ap)
Transcribed Image Text:Eco01091 20/4 Plai 48 PstAPI 179 Aath 2613 Liet 183 Sspl 2501 Ehel, SspDI 235 Pdmi 2234 Bogl 2215 Scal 2177 Tsol 2108 2456 MCS BsaX 659 pUC18/19 2686 bp NmeAlli 1822 Afl, Pscl 806 Gsul 1/84 Ctri0l 17/19, Eco311 1/66 Eam1105! I691 466 rep (PMB1) Cail 1217 Psp-i 1110 Figure 1 (https://www.fishersci.com/shop/products/thermo-scientific-puc18-pue19-dna-50-g/fersd0051) Figure I above is showing the plasmid map of PUC18. Discuss how screening is carried out if this plasmid is used for cloning. Explain the THREF. (3) important regions of this plasmid that enable it to work efficiently as a cloning vector? b) State the differences between the plasmid in Figure i with PBR322 in terms of their sercening procedures. c) Discuss TWO (2) methods of transformation of competent bacterial cells with the plasmid in Figure 1. d) bla (Ap)
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