EdIE Eca24 Sacl CTC TAG AGG 5 G TAA ANC GC GỌC CAG TCC CAA GCT TGC ATG CCT GCA CGA ACG TAC GA TAC CA AGC IGT 11c CIG 3' 3' C ATE TIG CrG GAG ATC TCC TAG GIA CCA GIA TCG ACA AAG GAC 5 Lacz - Ala Leu Ser Ala His Arg Cys Thr Ser Glu Leu Pro Asp Pro Val Ser Sar Ser le Vet Thr Met 1. You have determined that the amplicon you want to clone has enzyme restriction sites for Hindl!l and ECORI. After investigation you have seen that the pUC18/19 plasmid also have these enzyme restriction sites in its multiple cloning site (MCS on map above). After enzyme digestion your amplicon is 854 bp long. а. What length will the recombinant plasmid be after you have inserted your amplicon? Show your calculation. b. In the amplicon insert you have an enzyme restriction site for Ndel at 500 bp, If you digest the recombinant plasmid with this enzyme what length will the fragments be?
EdIE Eca24 Sacl CTC TAG AGG 5 G TAA ANC GC GỌC CAG TCC CAA GCT TGC ATG CCT GCA CGA ACG TAC GA TAC CA AGC IGT 11c CIG 3' 3' C ATE TIG CrG GAG ATC TCC TAG GIA CCA GIA TCG ACA AAG GAC 5 Lacz - Ala Leu Ser Ala His Arg Cys Thr Ser Glu Leu Pro Asp Pro Val Ser Sar Ser le Vet Thr Met 1. You have determined that the amplicon you want to clone has enzyme restriction sites for Hindl!l and ECORI. After investigation you have seen that the pUC18/19 plasmid also have these enzyme restriction sites in its multiple cloning site (MCS on map above). After enzyme digestion your amplicon is 854 bp long. а. What length will the recombinant plasmid be after you have inserted your amplicon? Show your calculation. b. In the amplicon insert you have an enzyme restriction site for Ndel at 500 bp, If you digest the recombinant plasmid with this enzyme what length will the fragments be?
Chapter6: Oral Medication Labels And Dosage Calculation
Section: Chapter Questions
Problem 9.2P
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Transcribed Image Text:Eco01091 2674 Pfol 46
BSTAPI 179
Aatll 2617
Ndel 183
Sspl 2501,
Ehel 235
Pdml 2294,
Bogl 2215.
Scal 2177
146/
236
lacz
2486
MCS
469-
PUC18/19
2686 bp
BsaXI 659
Sapl 683
852
AflI, Pscl 806
Gsul 1784.
1626
Cfr101 1779.
Eco311 1766.
Eam11051 1694
1466
rер (pMB1)
\BseYl 1110
\Cail 1217
Ecet Acc5
Eco24
MISJUC agerong pirer (20), 17-na
5' G TAA ANC GAC GGC CAG 1GC CAA GCT TGC ATG
CTC TAG AGG ATC CCC GGG TAC CGA GCT CGA ATT
AGC IGI 1IC CIG 3'
3' C ATI ITG CTG CCG G7C
GAG ATC
GIA CCA GIA TCG ACA AAG GAC 5
Lacz - Val Val Ala Leu Ala Leu Ser Ala His Arg Oys Thr Ser Glu Leu Pro Asp Gly Pro Val Ser Ser Ser
Vet Thr Met
1.
You have determined that the amplicon you want to clone has enzyme restriction sites for Hindlll and EcoRI. After
investigation you have seen that the pUC18/19 plasmid also have these enzyme restriction sites in its multiple cloning
site (MCS on map above). After enzyme digestion your amplicon is 854 bp long.
a.
What length will the recombinant plasmid be after you have inserted your amplicon? Show your calculation.
b.
In the amplicon insert you have an enzyme restriction site for Ndel at 500 bp, If you digest the recombinant plasmid
with this enzyme what length will the fragments be?
bla (Ap)
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