During the first successful gene therapy trial in which Ashanti DeSilva was treated for SCID, did the therapeutic gene delivered to Ashanti replace the defective copy of the ADA gene? Why were white blood cells chosen as the targets for the therapeutic gene?

Q: What is the definition of antibiotic resistance gene?

A: Antibiotics are the antimicrobial chemicals that can be bactericidal (kill the bacteria) or…

Q: What is the purpose of the targeting vector?

A: In molecular cloning, vector is a segment of DNA molecule, which is used as an artificial carrier of…

Q: My Avya mouse is brownish because a) the CpG island with the transposon is unmethylated. b) it was…

A: Epigenetic systems that facilitate gene-environment interactions are involved in the ability of…

Q: What is the molecular characteristic and phenotype of the silent mutation in multidrug resisitent…

A: Silent mutation are the mutations in DNA which do not have an phenotypic effect on the organism.…

Q: With regard to the CRISPR-Cas system that defends bacteriaagainst bacteriophage attack, what happens…

A: Bacteriophage are the viruses which infects bacteria.  CRISPR that is Clustered Regularly…

Q: All of the following apply to Luria and Delbrűck’s mutation theory as tested using E. Coli and T1…

A: The answer is option c.  All of the following apply to Luria and Delbrűck’s mutation theory as…

Q: Describe the method of pronuclear injection to generate transgenic mice?

A: Transgenic mice are produced by putting DNA in their genome from another source. There are a number…

Q: Define the therapeutic gene?

A: Gene is a functional unit of heredity. A gene is a sequence of nucleotides in genome that codes for…

Q: hat is the similarity of experimen vitamin c assay and the clearance of a molecule like glucose by…

A: A vitamin assay is the measurement of the activity or presence of a specific component in a specific…

Q: What are the two type of viral vector involved in gene therapy trials?

A: Viral vectors are the engineered tools which are used by molecular biologists to transfer genetic…

Q: How was it experimentally proven that the spacer sequences in the CRISPR locus were the elements…

A: CRISPR-cas system provides an adaptive defence mechanism to the prokaryotes which acquires…

Q: Can you suggest how we could have determined whether Bill’s aunts were carriers of the mutant BTK…

A: The Bruton tyrosine kinase is important for the growth and maturation of B cells by some change and…

Q: What is commonly used as a vector for introducing a DNA fragment in human lymphocytes?

A: Gene therapy is a method that allows the correction of the defective gene. In gene therapy, it is…

Q: (1) Why the ratio of PE/GFP fluorescence is measured in the FACS experiment, instead of measuring…

A: A G protein-coupled receptor (GPCR) helps control how much energy we have. The hormone called MC4R…

Q: What does MEGA stand for and what is this assay used to determine? How to do perform the assay?

A: Many assisted reproductive techniques are widely used around the globe.

Q: What distinguishes a retrovirus from an LTR transposon, and how is this difference enabled?

A: LTR retrotransposons are class I transposable elements characterized by the presence of long…

Q: The first person to receive gene therapy was a young girl with adenosine desminase(ADA) deficiency.…

A: Gene treatment endeavors to treat cancer or boost the body's capacity to combat illness by…

Q: What are the optimal conditions for EcoRV and Aval restriction enzymes?

A: Restriction enzymes are also known as molecular scissors since they are used in cutting specific…

Q: What is a notch 3 gene mutation test?

A: Notch is a cell surface receptor that receives and transmits signals in notch signalling pathway. It…

Q: How is “RFLP” technology used in the detection of “SNP” in sickle cell anemia? Please describe the…

A: RFLP is a technique used in molecular biology to detect differences in DNA sequences. The acronym…

Q: Describe how ES cells are used to generate knockoutmice.

A: Knockout mice is a genetically modified mice in which the researchers have inactivated or knocked…

Cancer Therapy