does because compounds can either intera mobile phase (i.e., solvent). Draw a picture th Iphenol and silica gel; and 2-phenylphenol a particles of SiO2 with silanol (Si-OH) groups

Q: Why are some of the detectors listed unsuitable for HPLC?

A: HPLC  is applicable for the use with solvent that has UV absorbance. A UV detector cannot be used…

Q: Which of the following is true about HPLC performance? * The smaller the internal diameter of the…

A:

Q: Calculate the Rf value for a spot in a TLC experiment if the solvent moved 14.9 cm and the spot…

A: Given values, Distance travelled by solvent = 14.9 cm Distance travelled by solute= 9.5 cm

Q: Identify some advantages and limitations of using HPLC, and explain

A: When organic mixtures of compounds are nonvolatile, thermally not stable, and have high molecular…

Q: (2) Set the wavelength of the spectrophotometer to 400 nm. . Place the stock solution (Solution 5)…

A: Each substance absorbs light at a particular wavelength rather than allowing all the light to…

Q: What is 2D gel electrophoresis

A: 2D gel electrophoresis is a technique for detection and separation of proteins. It is a combination…

Q: Show TWO (2) characteristics of the core enhanced technology that contribute to better separations,…

A: A question based on analytical separations that is to be accomplished.

Q: 4. Calculate the Rf of compound A in the TLC plate shown below. A 20 10 cm

A: Rf value, or retardation factor, is a ratio that describes the distance a component in a mixture…

Q: How can you vary the retention factor and selectivity factor?

A: Retention factor is a variable which indicates the time duration a component spends in the…

Q: Can an HPLC run be stopped during operation without impairing the separation

A: HPLC- High performance liquid chromatoghraphy It consists of two phase .One is stationary phase and…

Q: Column Plate Number: 16 Retention Factor: 3.1 Sample Peak Retention Time: 1.2minutes Sample Peak…

A: The objective of the question is to find the resolution and pressure if flow rate is adjusted to…

Q: Why is it that a warm cuvette does not lose any significant heat during the absorbance measurement…

A: Cuvettes are designed to hold samples specially for spectroscopic measurement.  Here the light beam…

Q: Demonstrate how to make TLC, and analyze the sample for any purpose, which deemed suitable for you

A: TLC is a thin layer chromatography, a technique used for separating non-volatile mixtures and…

Q: Broadening result in TLC is preferable. Select one: O True O False

A: An ideal chromatographic system would therefore produce peaks that were straight line spikes in…

Q: The solvent level in the development chamber should be above the level of the spotted compounds on…

A: The solvent level in the development chamber should be above the level of the spotted compounds on…

Q: How does the wavelength in chromatogram affect the signal intensity in an HPLC

A: wavelength in chromatogram affect the signal intensity in an HPLC explanation is given below.

Q: Your lab-mate observes a large amount of streaking and blurring of their samples when they visualize…

A: It is the major problem that YTLC plate seems to be blurred in the UV light.  But you can overcome…

Q: Transmitance 12 1.0 B 0.6 3 0.2 0.0 3500 3000 Fraction 11 Infrared Spectrum 2500 2000 Wavenumbers…

A: The objective of this question is to explain the Streching vibration peak present in the given…

Q: Interpret the IR spectrum.

A: In the IR spectrum, a sharp peak at 1600 cm-1 indicates the presence of aromatic C=C bond. The peak…

Q: 7. Alex accidentally drops a TLC plate into the jar, briefly submerging the whole plate in solvent…

A: It is possible that the TLC plate was damaged when it was dropped into the solvent. When a TLC plate…

Q: Chemistry Yes or No: Topic - HPLC 1.We need the void time so that we have a measure of the retention…

A: Concepts of HPLC.

Q: If a TLC was run in a solvent system that caused all the spots to show up at the baseline, how could…

A: Rf value Vs polarity of the eluent

Q: It is acceptable to draw the TLC spotting line with a pen as long as it is marked very lightly.…

A: False

Q: 3295 IR Spectrum (liquid film) 4000 3000 2114 2000 m nnt 1600 (cm¹) 1200 800

A: The objective of the question is to interpret the given Infrared (IR) spectrum values and determine…

Q: Can you TYPE the definitions of the all of words please Chromatography TLC Stationary phase Mobile…

A: Most of the time, the compounds we encounter are not in their purest form. They are essentially a…

Q: Find term sy mbel for the following:- 3+ 3 - Mn 7 Sc, N,P

A: Given Element = Mn2+  ,Sc3+  ,N  ,P3-     Element                          Number of electron…

Q: How does the retention time of a compound change with separation conditions? Is this change linear…

A: The interval between the injection of a sample and the detection of substances in that sample is…

Q: You can perform a TLC experiment even if the solvent level is above the level of the initial spots.…

A: Here I have solved the question

Q: Calculate the RF value for a spot in a TLC experiment if the solvent move 13.5 cm and the spot move…

A:

Q: During TLC, what is the stationary phase and what is the mobile phase?

A: Chromatography is a laboratory technique for the separation of a mixture into its components. It is…

Q: Compare and contrast GC/MS IR spectrophotometry. TLC spectroscopy, and UV/VIS spectrophotometry Make…

A: Analytical spectroscopy:

Q: How would the resolution (separation into discrete peaks) be affected if you used a column that was…

A: Resolution depends on column lengths and therefore it will be affrcted by the decrease in length of…

Q: In general, increasing the molecular weight of a Gd-based MR contrast agent results in an increase…

A: True. The proton relaxivity of a Gd(III) compound is determined by several factors and can be varied…

Q: For each of the following statements, answer TRUE or FALSE and explain your answer briefly.   It…

A: True Potassium Dichromate solutions (235 - 430 nm)The most widely recognised reference material for…

Q: In HPLC, why is purging or initialization being done before analysis of samples, explain.

A: ✓basically following two reasons for using purging and initialization during HPLC sample preparation…

Q: What is the retention factor of a sample when after injectiion the void time (tM) took 4.18 sec? The…

A:

Q: Compare gradient and isocratic mode in HPLC.

A: Gradient and isocratic mode in HPLC are the two different modes of operating condition in HPLC.

Q: How does increasing the polarity of the developing solvent affect the Rf of a compound on a silica…

A: TLC stands for Thin Layer Chromatography. This process is basically used for the differentiation of…

Q: The aspirin we made in lab gad 125-133 melting point and I attached the IR. Base on this…

A: The structure of aspirin is: It has the following major peaks in IR spectrum: vOH = 3100-3300 cm-1…

Q: Explain why it is needed to filter the solvent before introducing it to the HPLC system

A: Basically there are following two reasons for using filtration during HPLC sample preparation are…

Q: Question 19 In AFM, what is the consequence of low scanning time? limited magnification thermal…

A: AFM refers to Atomic Force Microscopy or Scanning force Microscopy.  It is a high resolution type of…

Micelles and Biological Membrane

Micelles are the type of lipid molecules that organize themselves in spherical shape in aqueous solutions. The production of the micelle is a reply to the amphipathic behavior of fatty acids. It means that they consist of both hydrophobic regions and hydrophilic regions. In micelles, polar head groups (hydrophilic groups) produce an outer layer associated with water whereas nonpolar tails (hydrophobic groups) are organized in the interior.