(DNMP), (DNMP), DNA polymerase I P-P DNTP Mg2+ (DNMP), (DNMP), +1 Deoxyribonucleoside triphosphates (ДATP, dTTP, GCТР, dGTP) DNA template (DNMP), and a portion of its Inorganic pyrophosphate Complement to template strand is extended by one nucleotide (n + 1) complement (DNMP), FIGURE 11.7 The chemical reaction catalyzed by DNA polymerase I. During each step, a single nucleotide is added to the growing complement of the DNA template, using a nucleoside triphosphate as the substrate. The release of inorganic pyrophosphate drives the reaction energetically.

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What is the role of Mg2+ in this reaction?

(DNMP),
(DNMP),
DNA
polymerase I
P-P
DNTP
Mg2+
(DNMP),
(DNMP), +1
Deoxyribonucleoside
triphosphates
(ДATP, dTTP, GCТР, dGTP)
DNA template (DNMP),
and a portion of its
Inorganic
pyrophosphate
Complement to template
strand is extended by one
nucleotide (n + 1)
complement (DNMP),
FIGURE 11.7 The chemical reaction catalyzed by DNA polymerase I. During each step, a single
nucleotide is added to the growing complement of the DNA template, using a nucleoside
triphosphate as the substrate. The release of inorganic pyrophosphate drives the reaction
energetically.
Transcribed Image Text:(DNMP), (DNMP), DNA polymerase I P-P DNTP Mg2+ (DNMP), (DNMP), +1 Deoxyribonucleoside triphosphates (ДATP, dTTP, GCТР, dGTP) DNA template (DNMP), and a portion of its Inorganic pyrophosphate Complement to template strand is extended by one nucleotide (n + 1) complement (DNMP), FIGURE 11.7 The chemical reaction catalyzed by DNA polymerase I. During each step, a single nucleotide is added to the growing complement of the DNA template, using a nucleoside triphosphate as the substrate. The release of inorganic pyrophosphate drives the reaction energetically.
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