Bacterial- DNA fragment (no origin of replication) chromosome DNA molecules without an origin of replication cannot replicate in a cell. Only one daughter cell will have a copy of the DNA fragment. (a) Non-integrated DNA fragment DNA fragment (no origin of replication) Homologous recombination A DNA fragment integrated into a bacterial chromosome can be replicated and passed on to daughter cells. All daughter cells will have a copy of the DNA fragment. (b) Integrated DNA fragment FIGURE 8.18 DNA Must Be Part of a Replicon to Be Malntalned In a Population (a) DNA without an orlgln of replicatlon wll not be passed on to any new daughter cells during growth of the populatlon. (b) If the DNA becomes Integrated within a replicon of the cell, It will be Inherlted by all daughter cells. 00

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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If the DNA fragment encodes penicillin resistance, how could you experimentally distinguish between (a) and (b)?

Bacterial-
DNA fragment
(no origin of replication)
chromosome
DNA molecules without an origin
of replication cannot replicate in a cell.
Only one daughter cell
will have a copy of the
DNA fragment.
(a) Non-integrated DNA fragment
DNA fragment
(no origin of replication)
Homologous recombination
A DNA fragment integrated into a
bacterial chromosome can be replicated
and passed on to daughter cells.
All daughter cells
will have a copy of the
DNA fragment.
(b) Integrated DNA fragment
FIGURE 8.18 DNA Must Be Part of a Replicon to Be Malntalned In a Population (a) DNA without an orlgln of replicatlon wll not be passed
on to any new daughter cells during growth of the populatlon. (b) If the DNA becomes Integrated within a replicon of the cell, It will be Inherlted by
all daughter cells.
00
Transcribed Image Text:Bacterial- DNA fragment (no origin of replication) chromosome DNA molecules without an origin of replication cannot replicate in a cell. Only one daughter cell will have a copy of the DNA fragment. (a) Non-integrated DNA fragment DNA fragment (no origin of replication) Homologous recombination A DNA fragment integrated into a bacterial chromosome can be replicated and passed on to daughter cells. All daughter cells will have a copy of the DNA fragment. (b) Integrated DNA fragment FIGURE 8.18 DNA Must Be Part of a Replicon to Be Malntalned In a Population (a) DNA without an orlgln of replicatlon wll not be passed on to any new daughter cells during growth of the populatlon. (b) If the DNA becomes Integrated within a replicon of the cell, It will be Inherlted by all daughter cells. 00
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