Discuss the composition of the transition state for the formation of ATP by ATP Synthase. a) Where is the active site for this enzyme located? b) How are the amino acid side chains from the α and β subunits of ATP Synthase involved? c) Discuss the importance of Mg+2 in the mechanism of this enzyme.
Discuss the composition of the transition state for the formation of ATP by ATP Synthase.
a) Where is the active site for this enzyme located?
b) How are the amino acid side chains from the α and β subunits of ATP Synthase involved?
c) Discuss the importance of Mg+2 in the mechanism of this enzyme.
The formation of ATP (adenosine triphosphate) from ADP (adenosine diphosphate) and inorganic phosphate is under the control of the enzyme ATP synthase. ATP synthesis occurs in the eukaryotic inner mitochondrial membrane or prokaryotic plasma membrane and plant thylakoid membrane (in chloroplasts), where the proton motive force is coupled to ATP synthesis. The electrochemical gradient of the electron transport chain in aerobic respiration is used to generate the proton motive force.
The transition state is an intermediate in the reaction mechanism. It is an unstable structure that breaks down into the reaction substrates or the products. The transition state has higher energy than the substrates or products. This creates an energy barrier called the activation energy. Enzymes help in catalysis by lowering the activation energy.
Step by step
Solved in 2 steps
