SERIAL DILUTIONDilution is the act of making a weaker solution from a stronger one. This is usually done by adding a wateror saline, which contains none of the material being diluted. Dilution is usually expressed as one unit of theoriginal solution to the total number of units of final solution. Serial dilution means decreasing the volumeof serum progressively by maintaining a constant volume of fluid. Most commonly, serial dilutions aretwofold, that is, each dilution is half as concentrated as the preceding one. The total volume in each tube isthe same.Principle:Serial dilutions are a set of dilutions in which the dilution factor is exactly the same at each step. These areused to make high dilutions with a small number of test tubes and a minimal amount of diluent. This iscommonly done to determine the strength or tier of a particular antibody in patient serumdiagnosis of a disease state. Traditionally, serological pipettes have been used in this process, but now it ismore common to employ micropipettes for this purpose. In this experiment, a series of doubling dilutionwill be made with both serological pipettes and micropipettes, and the results will be compared.a part of thePreparation of Two - Fold Serial DilutionTest Tube1234Saline (ml)Patientserum (ml)0.10.10.10.1 of 1:20.10.1 of 1:40.10.10.1 of 1:80.1 of 1:16Mix anddiscard 0.11:32Final1:21:41:81:16DilutionCreate an illustration showing the preparation of two-fold serial dilution. Use different colors todifferentiate the NSS from the serum. Your illustration should follow the ratio of the final dilutions asshown in the table above.

Dilution is the process of decreasing the concentration of a particular solute or solvent.

Dilution is the act of making a weaker solution from a stronger one. This is usually done by adding a water or saline, which contains none of the material being diluted. Dilution is usually expressed as one unit of the original solution to the total number of units of final solution. Serial dilution means decreasing the volume of serum progressively by maintaining a constant volume of fluid. Most commonly, serial dilutions are twofold, that is, each dilution is half as concentrated as the preceding one. The total volume in each tube is the same.

Dilution is the act of making a weaker solution from a stronger one. This is usually done by adding a water or saline, which contains none of the material being diluted. Dilution is usually expressed as one unit of the original solution to the total number of units of final solution. Serial dilution means decreasing the volume of serum progressively by maintaining a constant volume of fluid. Most commonly, serial dilutions are twofold, that is, each dilution is half as concentrated as the preceding one. The total volume in each tube is the same.

Microbiology for Surgical Technologists (MindTap Course List)

2nd Edition

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Margaret Rodriguez, Paul Price

Chapter6: Microbial Viability And Growth

Section: Chapter Questions

Problem 1UTM: Dr. Martinez ordered a Foley catheter for his patient who was on corticosteroids after kidney...

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