Determine the subunit composition of this protein (i.e., how many subunits are in its quaternary structure?). MM SDS SDS

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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SDS-AGE analysis of protein for subunit composition: A protein has a molecular mass of 400 kDa when
measured by size-exclusion chromatography (gel filtration). When subjected to gel electrophoresis in the
presence of SDS only and SDS plus 2-ME, the following pattern of SDS-PAGE is observed. MM - molecular
standard proteins with known molecular weight (in kDa) as shown on the left of the gel are used to determine
the size of unknown proteins.
a) Determine the subunit composition of this protein (i.e., how many subunits are in its quaternary structure?).
MM
SDS
SDS
2-ME
kDa
180-
160-
100-
90-
60-
50-
Transcribed Image Text:SDS-AGE analysis of protein for subunit composition: A protein has a molecular mass of 400 kDa when measured by size-exclusion chromatography (gel filtration). When subjected to gel electrophoresis in the presence of SDS only and SDS plus 2-ME, the following pattern of SDS-PAGE is observed. MM - molecular standard proteins with known molecular weight (in kDa) as shown on the left of the gel are used to determine the size of unknown proteins. a) Determine the subunit composition of this protein (i.e., how many subunits are in its quaternary structure?). MM SDS SDS 2-ME kDa 180- 160- 100- 90- 60- 50-
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