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Determination of Glucose The Nelson method for determining glucose can be used to determine glucose content of blood; however, it is necessary to first remove the proteins from the blood since they interfere with the determination. This can be done by adding appropriate amounts of protein precipitating agents to the blood, filtering the mixture to obtain the so-called protein-free filtrate. Assume that your unknown represented a protein-free filtrate prepared in the following manner; to 1 mL of whole blood you added 15 mL of water followed by 2 mL of barium hydroxide and then 2 mL of zinc sulfate. This mixture is then filtered to give a protein-free filtrate. Assume that your unknown represents an aliquot of this filtrate and calculate the amount of glucose present in 100 mL of the original whole blood. Show work. mg/dL of glucose

Determination of Glucose The Nelson method for determining glucose can be used to determine glucose content of blood; however, it is necessary to first remove the proteins from the blood since they interfere with the determination. This can be done by adding appropriate amounts of protein precipitating agents to the blood, filtering the mixture to obtain the so-called protein-free filtrate. Assume that your unknown represented a protein-free filtrate prepared in the following manner; to 1 mL of whole blood you added 15 mL of water followed by 2 mL of barium hydroxide and then 2 mL of zinc sulfate. This mixture is then filtered to give a protein-free filtrate. Assume that your unknown represents an aliquot of this filtrate and calculate the amount of glucose present in 100 mL of the original whole blood. Show work. mg/dL of glucose

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Determination of Glucose The Nelson method for determining glucose can be used to determine glucose content of blood; however, it is necessary to first remove the proteins from the blood since they interfere with the determination. This can be done by adding appropriate amounts of protein precipitating agents to the blood, filtering the mixture to obtain the so-called protein-free filtrate. Assume that your unknown represented a protein-free filtrate prepared in the following manner; to 1 mL of whole blood you added 15 mL of water followed by 2 mL of barium hydroxide and then 2 mL of zinc sulfate. This mixture is then filtered to give a protein-free filtrate. Assume that your unknown represents an aliquot of this filtrate and calculate the amount of glucose present in 100 mL of the original whole blood. Show work. mg/dL of glucose
Transcribed Image Text:Determination of Glucose The Nelson method for determining glucose can be used to determine glucose content of blood; however, it is necessary to first remove the proteins from the blood since they interfere with the determination. This can be done by adding appropriate amounts of protein precipitating agents to the blood, filtering the mixture to obtain the so-called protein-free filtrate. Assume that your unknown represented a protein-free filtrate prepared in the following manner; to 1 mL of whole blood you added 15 mL of water followed by 2 mL of barium hydroxide and then 2 mL of zinc sulfate. This mixture is then filtered to give a protein-free filtrate. Assume that your unknown represents an aliquot of this filtrate and calculate the amount of glucose present in 100 mL of the original whole blood. Show work. mg/dL of glucose
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