Describe the preparation of 0.50L of a 0.20 M Hepes buffer pH 7.7 You are given the following materials in lab: Hepes: pKa 7.55; Molar mass: 260.29 g/mole, conjugate base, solid. 1.0 M HCI , 1.0 M NAOH, and dH2O available to use (you have to decide if you need to titrate with HCI or NaOH).

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Describe the preparation of 0.50L of a
0.20 M Hepes buffer pH 7.7
You are given the following materials in
lab:
Hepes: pka 7.55; Molar mass: 260.29
g/mole, conjugate base, solid.
1.0 M HCI , 1.0 M NAOH, and dH2O
available to use (you have to decide if
you need to titrate with HCI or NaOH).
Transcribed Image Text:Describe the preparation of 0.50L of a 0.20 M Hepes buffer pH 7.7 You are given the following materials in lab: Hepes: pka 7.55; Molar mass: 260.29 g/mole, conjugate base, solid. 1.0 M HCI , 1.0 M NAOH, and dH2O available to use (you have to decide if you need to titrate with HCI or NaOH).
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