Homework Help is Here – Start Your Trial Now!
Engineering
Chemical Engineering
Describe how an efficient separation of anions can be achieved using capillary zone electrophoresis (CZE). Two neutral compounds, A and B are succesfull separated by micellar electrokinetic chromatography (MEKC) using 15 mM borate buffer (pH 8) with 50 mM sodium dodecyl sulphate (SDS). i) The migration time of A and B are 8.1 and 13.0 min, respectively. Explain the hydrophobicity property of the compound based on the migration times obtained. ii) If SDS is added in a very low concentration (< 50 mM) into the system, explain the obtained separation. Give an advantage of MEKC over CZE.

Describe how an efficient separation of anions can be achieved using capillary zone electrophoresis (CZE). Two neutral compounds, A and B are succesfull separated by micellar electrokinetic chromatography (MEKC) using 15 mM borate buffer (pH 8) with 50 mM sodium dodecyl sulphate (SDS). i) The migration time of A and B are 8.1 and 13.0 min, respectively. Explain the hydrophobicity property of the compound based on the migration times obtained. ii) If SDS is added in a very low concentration (< 50 mM) into the system, explain the obtained separation. Give an advantage of MEKC over CZE.

Introduction to Chemical Engineering Thermodynamics
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
See similar textbooks
icon
Related questions
Question
Describe how an efficient separation of anions can be achieved using capillary zone electrophoresis (CZE). a) b) Two neutral compounds, A and B are succesfull separated by micellar electrokinetic chromatography (MEKC) using 15 mM borate buffer (pH 8) with 50 mM sodium dodecyl sulphate (SDS). i) The migration time of A and B are 8.1 and 13.0 min, respectively. Explain the hydrophobicity property of the compound based on the migration times obtained. i) If SDS is added in a very low concentration (< 50 mM) into the system, explain the obtained separation. c) Give an advantage of MEKC over CZE.
Transcribed Image Text:Describe how an efficient separation of anions can be achieved using capillary zone electrophoresis (CZE). a) b) Two neutral compounds, A and B are succesfull separated by micellar electrokinetic chromatography (MEKC) using 15 mM borate buffer (pH 8) with 50 mM sodium dodecyl sulphate (SDS). i) The migration time of A and B are 8.1 and 13.0 min, respectively. Explain the hydrophobicity property of the compound based on the migration times obtained. i) If SDS is added in a very low concentration (< 50 mM) into the system, explain the obtained separation. c) Give an advantage of MEKC over CZE.
Expert Solution
steps

Step by step

Solved in 6 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Recommended textbooks for you
Introduction to Chemical Engineering Thermodynami…
Introduction to Chemical Engineering Thermodynami…
Chemical Engineering
ISBN:
9781259696527
Author:
J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:
McGraw-Hill Education
Elementary Principles of Chemical Processes, Bind…
Elementary Principles of Chemical Processes, Bind…
Chemical Engineering
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY
Elements of Chemical Reaction Engineering (5th Ed…
Elements of Chemical Reaction Engineering (5th Ed…
Chemical Engineering
ISBN:
9780133887518
Author:
H. Scott Fogler
Publisher:
Prentice Hall
Process Dynamics and Control, 4e
Process Dynamics and Control, 4e
Chemical Engineering
ISBN:
9781119285915
Author:
Seborg
Publisher:
WILEY
Industrial Plastics: Theory and Applications
Industrial Plastics: Theory and Applications
Chemical Engineering
ISBN:
9781285061238
Author:
Lokensgard, Erik
Publisher:
Delmar Cengage Learning
Unit Operations of Chemical Engineering
Unit Operations of Chemical Engineering
Chemical Engineering
ISBN:
9780072848236
Author:
Warren McCabe, Julian C. Smith, Peter Harriott
Publisher:
McGraw-Hill Companies, The
SEE MORE TEXTBOOKS