Describe a way to determine the rate of the alcohol dehydrogenase catalyzed oxidation of ethanol by NAD*. O Acetaldehyde is formed as a product; it absorbs light at 340 nm. Therefore, the rate of the oxidation reaction can be determined by monitoring the increase in absorbance at 340 nm as a function of time. O Ethanol is a reactant; it absorbs light at 340 nm. Therefore, the rate of the oxidation reaction can be determined by monitoring the decrease in absorbance at 340 nm as a function of time. O NAD* is a reactant; it absorbs light at 340 nm. Therefore, the rate of the oxidation reaction can be determined by monitoring the decrease in absorbance at 340 nm as a function of time, o NADH is formed as a product; it absorbs light at 340 nm. Therefore, the rate of the oxidation reaction can be determined by monitoring the increase in absorbance at 340 nm as a function of time.
Catalysis and Enzymatic Reactions
Catalysis is the kind of chemical reaction in which the rate (speed) of a reaction is enhanced by the catalyst which is not consumed during the process of reaction and afterward it is removed when the catalyst is not used to make up the impurity in the product. The enzymatic reaction is the reaction that is catalyzed via enzymes.
Lock And Key Model
The lock-and-key model is used to describe the catalytic enzyme activity, based on the interaction between enzyme and substrate. This model considers the lock as an enzyme and the key as a substrate to explain this model. The concept of how a unique distinct key only can have the access to open a particular lock resembles how the specific substrate can only fit into the particular active site of the enzyme. This is significant in understanding the intermolecular interaction between proteins and plays a vital role in drug interaction.
The oxidation of alcohol using NAD+ and the study of the rate of alcohol dehydrogenase can be done by spectrophotometer. NADH formed has a specific absorbance in the UV spectrum region. Thus by monitoring the reaction by absorbance measurement one can find the rate of alcohol dehydrogenase reaction easily.
So the correct answer is D
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