d. The chromatogram below was the first attempt at separating this four-component mixture. This initial separation was carried out using a mobile phase of 50% methanol/50% water. What was changed about the method to achieve the chromatogram shown above (full separation)? Why did this change lead to an improved separation?

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d. The chromatogram below was the first attempt at separating this four-component
mixture. This initial separation was carried out using a mobile phase of 50%
methanol/50% water. What was changed about the method to achieve the
chromatogram shown above (full separation)? Why did this change lead to an improved
separation?
50% MeOH
Transcribed Image Text:d. The chromatogram below was the first attempt at separating this four-component mixture. This initial separation was carried out using a mobile phase of 50% methanol/50% water. What was changed about the method to achieve the chromatogram shown above (full separation)? Why did this change lead to an improved separation? 50% MeOH
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