d. The chromatogram below was the first attempt at separating this four-component mixture. This initial separation was carried out using a mobile phase of 50% methanol/50% water. What was changed about the method to achieve the chromatogram shown above (full separation)? Why did this change lead to an improved separation?
d. The chromatogram below was the first attempt at separating this four-component mixture. This initial separation was carried out using a mobile phase of 50% methanol/50% water. What was changed about the method to achieve the chromatogram shown above (full separation)? Why did this change lead to an improved separation?
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:d. The chromatogram below was the first attempt at separating this four-component
mixture. This initial separation was carried out using a mobile phase of 50%
methanol/50% water. What was changed about the method to achieve the
chromatogram shown above (full separation)? Why did this change lead to an improved
separation?
50% MeOH
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