d). It's the temperature at which the mass of single stranded DNA equals the mass of double stranded DNA.
d). It's the temperature at which the mass of single stranded DNA equals the mass of double stranded DNA.
Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![Question:
Which of the following statements is NOT a correct description of the DNA melting
temperature for a system in which we have equal molar concentrations of two
distinguishable strands?
a). It's the temperature at which the molar concentrations of the strands (S₁ and S₂)
and the duplex (D) are related by the following equation:
[S1] + [S2] = 2[D].
b). It's the temperature at which half the DNA strands have associated with one
another to form duplex DNA.
c). It's the temperature at which the absorption at 260 nm is midway between that of
single-stranded DNA and that of double-stranded DNA.
d). It's the temperature at which the mass of single stranded DNA equals the mass of
double stranded DNA.
e). It's the temperature at which the equilibrium constant for duplex formation is the
inverse of the total strand concentration times 4.
f). None of the above (i.e., all the above are correct descriptions of the DNA melting
temperature for a system in which we have equal molar concentrations of two
distinguishable strands).
O a
O b
C
Od
The definition of melting temperature depends upon the relative concentrations
of the two strands. In a phase transition (e.g., the water/ice transition), the
standard free energy change is O at the melting temperature. This is not true for
the DNA melting transition as is apparent from the fact that the equilibrium
constant at the melting temperature is not 1.
e
Of
Oboth a and b](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F33a1b850-bf77-4f24-8dca-6f385fdd0e5e%2F34960182-7e59-4728-b960-0e4c51d616cc%2Fxjc68ln_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Question:
Which of the following statements is NOT a correct description of the DNA melting
temperature for a system in which we have equal molar concentrations of two
distinguishable strands?
a). It's the temperature at which the molar concentrations of the strands (S₁ and S₂)
and the duplex (D) are related by the following equation:
[S1] + [S2] = 2[D].
b). It's the temperature at which half the DNA strands have associated with one
another to form duplex DNA.
c). It's the temperature at which the absorption at 260 nm is midway between that of
single-stranded DNA and that of double-stranded DNA.
d). It's the temperature at which the mass of single stranded DNA equals the mass of
double stranded DNA.
e). It's the temperature at which the equilibrium constant for duplex formation is the
inverse of the total strand concentration times 4.
f). None of the above (i.e., all the above are correct descriptions of the DNA melting
temperature for a system in which we have equal molar concentrations of two
distinguishable strands).
O a
O b
C
Od
The definition of melting temperature depends upon the relative concentrations
of the two strands. In a phase transition (e.g., the water/ice transition), the
standard free energy change is O at the melting temperature. This is not true for
the DNA melting transition as is apparent from the fact that the equilibrium
constant at the melting temperature is not 1.
e
Of
Oboth a and b
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