Construct a Lineweaver-Burk plot to answer the following questions: (a) What are the apparent KM and Vmax values in the presence and absence of the inhibitor? (b) What kind of an inhibitor is threo-sphingosine? Explain.
Construct a Lineweaver-Burk plot to answer the following questions: (a) What are the apparent KM and Vmax values in the presence and absence of the inhibitor? (b) What kind of an inhibitor is threo-sphingosine? Explain.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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![3. Sphingosine-1-phosphate (SPP) is important for cell survival. The synthesis of SPP from
sphingosine and ATP is catalyzed by the enzyme sphingosine kinase. The velocity of the
sphingosine kinase reaction was measured in the presence and absence of threo-sphingosine, a
stereoisomer of sphingosine that inhibits the enzyme. The results are shown below.
4
[S] (mM)
Vo No Inhibitor (mM min)
Vo With Inhibitor (mM min)
3
4.58
3.66
6.4
5.12
7
7.72
6.18
9
8.72
6.98
11
9.5
7.6
Construct a Lineweaver-Burk plot to answer the following questions:
(a) What are the apparent KM and Vmax values in the presence and absence of the inhibitor?
(b) What kind of an inhibitor is threo-sphingosine? Explain.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc61217c1-2079-42bf-a218-52fe015333ee%2F7f0734aa-7d68-4fc4-8e53-0cae3341c294%2F03mim17_processed.jpeg&w=3840&q=75)
Transcribed Image Text:3. Sphingosine-1-phosphate (SPP) is important for cell survival. The synthesis of SPP from
sphingosine and ATP is catalyzed by the enzyme sphingosine kinase. The velocity of the
sphingosine kinase reaction was measured in the presence and absence of threo-sphingosine, a
stereoisomer of sphingosine that inhibits the enzyme. The results are shown below.
4
[S] (mM)
Vo No Inhibitor (mM min)
Vo With Inhibitor (mM min)
3
4.58
3.66
6.4
5.12
7
7.72
6.18
9
8.72
6.98
11
9.5
7.6
Construct a Lineweaver-Burk plot to answer the following questions:
(a) What are the apparent KM and Vmax values in the presence and absence of the inhibitor?
(b) What kind of an inhibitor is threo-sphingosine? Explain.
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