Consider an enzyme that uses general acid-base catalysis such that the reverse protonated form (call it HE) functions in one direction of the reaction, and the typically protonated form (call it EH) functions in the other direction of the reaction. Both the doubly protonated (call it HEH) and doubly unprotonated (call it E) forms of the enzyme are not effective in catalyzing either direction of the reaction. If the first macroscopic pk, of this enzyme (call it pK,) is 6.96. the second macroscopic pK, of this enzyme (call it pK2) is 8.74, and one of the microscopic pK,s (pKA in the scheme below) is 7.77, then what proportion of the enzyme would be in the HEH form at pH 7.52? Enter your answer as a percent to the nearest tenth.
Consider an enzyme that uses general acid-base catalysis such that the reverse protonated form (call it HE) functions in one direction of the reaction, and the typically protonated form (call it EH) functions in the other direction of the reaction. Both the doubly protonated (call it HEH) and doubly unprotonated (call it E) forms of the enzyme are not effective in catalyzing either direction of the reaction. If the first macroscopic pk, of this enzyme (call it pK,) is 6.96. the second macroscopic pK, of this enzyme (call it pK2) is 8.74, and one of the microscopic pK,s (pKA in the scheme below) is 7.77, then what proportion of the enzyme would be in the HEH form at pH 7.52? Enter your answer as a percent to the nearest tenth.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Concept explainers
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
Question
100%
correct answer should be 20.6 but how?
Transcribed Image Text:Consider an enzyme that uses general acid-base
catalysis such that the reverse protonated form (call it
HE) functions in one direction of the reaction, and
the typically protonated form (call it EH) functions in
the other direction of the reaction. Both the doubly
protonated (call it HEH) and doubly unprotonated
(call it E) forms of the enzyme are not effective in
catalyzing either direction of the reaction. If the first
macroscopic pk, of this enzyme (call it pK1) is 6.96,
the second macroscopic pK, of this enzyme (call it
pK2) is 8.74, and one of the microscopic pK,s (pKA in
the scheme below) is 7.77, then what proportion of
the enzyme would be in the HEH form at pH 7.52?
Enter your answer as a percent to the nearest tenth.
НЕН
KB
H
EH
НЕ
Kc
H
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.Recommended textbooks for you
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON