Biology: The Unity and Diversity of Life (MindTap Course List)
15th Edition
ISBN:9781337408332
Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Chapter36: Circulation
Section: Chapter Questions
Problem 3CT
Related questions
Question
Comparing chlorophyll a and b , which of the two traveled furthest up the tlc plate ? Why is that ?
![1. Draw a simple sketch of your TLC plate to the right, indicating the location of the bands
you see and which pigments you think they are.
yellow
in centim 1o
besit
The Retardation Factor (R) is a way to normalize the separation of substances among different
TLC plates using the same developing solution, and gives the user a relative difference in the
separation of the substances. It is calculated as:
green
demo
dark
icar
distance pig menahter
travels from
Start torfinish
tion
Rf
distance from line to spot
yellow
CC
%3D
distance from line to developing solution front
distance the
solvent Fravels
2. Using a ruler calculate the Rf values for your separated pigments and log them into the
table below, starting with the pigment closest to the solvent front (top).
Light
green
Pigment Rf values
Pigments
R/ value
Suspected name of pigment
4.516.5
69
4.176.5
3.3/6.5
chlorophyll A
chlorophy 1l A
chlorojphyll B
,63
51
|3D](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F9b0e7686-0c0d-4eba-ac81-54e736b47f2f%2Fcc5d0c9c-0b73-4dd9-a575-da6b16d7d473%2F9n27y8i.jpeg&w=3840&q=75)
Transcribed Image Text:1. Draw a simple sketch of your TLC plate to the right, indicating the location of the bands
you see and which pigments you think they are.
yellow
in centim 1o
besit
The Retardation Factor (R) is a way to normalize the separation of substances among different
TLC plates using the same developing solution, and gives the user a relative difference in the
separation of the substances. It is calculated as:
green
demo
dark
icar
distance pig menahter
travels from
Start torfinish
tion
Rf
distance from line to spot
yellow
CC
%3D
distance from line to developing solution front
distance the
solvent Fravels
2. Using a ruler calculate the Rf values for your separated pigments and log them into the
table below, starting with the pigment closest to the solvent front (top).
Light
green
Pigment Rf values
Pigments
R/ value
Suspected name of pigment
4.516.5
69
4.176.5
3.3/6.5
chlorophyll A
chlorophy 1l A
chlorojphyll B
,63
51
|3D
Expert Solution
![](/static/compass_v2/shared-icons/check-mark.png)
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
![Blurred answer](/static/compass_v2/solution-images/blurred-answer.jpg)
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Recommended textbooks for you
![Biology: The Unity and Diversity of Life (MindTap…](https://www.bartleby.com/isbn_cover_images/9781337408332/9781337408332_smallCoverImage.gif)
Biology: The Unity and Diversity of Life (MindTap…
Biology
ISBN:
9781337408332
Author:
Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:
Cengage Learning
![Anatomy & Physiology](https://www.bartleby.com/isbn_cover_images/9781938168130/9781938168130_smallCoverImage.gif)
Anatomy & Physiology
Biology
ISBN:
9781938168130
Author:
Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
Publisher:
OpenStax College
![Cardiopulmonary Anatomy & Physiology](https://www.bartleby.com/isbn_cover_images/9781337794909/9781337794909_smallCoverImage.jpg)
Cardiopulmonary Anatomy & Physiology
Biology
ISBN:
9781337794909
Author:
Des Jardins, Terry.
Publisher:
Cengage Learning,
![Biology: The Unity and Diversity of Life (MindTap…](https://www.bartleby.com/isbn_cover_images/9781337408332/9781337408332_smallCoverImage.gif)
Biology: The Unity and Diversity of Life (MindTap…
Biology
ISBN:
9781337408332
Author:
Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:
Cengage Learning
![Anatomy & Physiology](https://www.bartleby.com/isbn_cover_images/9781938168130/9781938168130_smallCoverImage.gif)
Anatomy & Physiology
Biology
ISBN:
9781938168130
Author:
Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
Publisher:
OpenStax College
![Cardiopulmonary Anatomy & Physiology](https://www.bartleby.com/isbn_cover_images/9781337794909/9781337794909_smallCoverImage.jpg)
Cardiopulmonary Anatomy & Physiology
Biology
ISBN:
9781337794909
Author:
Des Jardins, Terry.
Publisher:
Cengage Learning,
![Fundamentals of Sectional Anatomy: An Imaging App…](https://www.bartleby.com/isbn_cover_images/9781133960867/9781133960867_smallCoverImage.gif)
Fundamentals of Sectional Anatomy: An Imaging App…
Biology
ISBN:
9781133960867
Author:
Denise L. Lazo
Publisher:
Cengage Learning