Comparing chlorophyll a and b , which of the two traveled furthest up the tlc plate ? Why is that ?

Biology: The Unity and Diversity of Life (MindTap Course List)
15th Edition
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Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
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Chapter36: Circulation
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Comparing chlorophyll a and b , which of the two traveled furthest up the tlc plate ? Why is that ? 

1. Draw a simple sketch of your TLC plate to the right, indicating the location of the bands
you see and which pigments you think they are.
yellow
in centim 1o
besit
The Retardation Factor (R) is a way to normalize the separation of substances among different
TLC plates using the same developing solution, and gives the user a relative difference in the
separation of the substances. It is calculated as:
green
demo
dark
icar
distance pig menahter
travels from
Start torfinish
tion
Rf
distance from line to spot
yellow
CC
%3D
distance from line to developing solution front
distance the
solvent Fravels
2. Using a ruler calculate the Rf values for your separated pigments and log them into the
table below, starting with the pigment closest to the solvent front (top).
Light
green
Pigment Rf values
Pigments
R/ value
Suspected name of pigment
4.516.5
69
4.176.5
3.3/6.5
chlorophyll A
chlorophy 1l A
chlorojphyll B
,63
51
|3D
Transcribed Image Text:1. Draw a simple sketch of your TLC plate to the right, indicating the location of the bands you see and which pigments you think they are. yellow in centim 1o besit The Retardation Factor (R) is a way to normalize the separation of substances among different TLC plates using the same developing solution, and gives the user a relative difference in the separation of the substances. It is calculated as: green demo dark icar distance pig menahter travels from Start torfinish tion Rf distance from line to spot yellow CC %3D distance from line to developing solution front distance the solvent Fravels 2. Using a ruler calculate the Rf values for your separated pigments and log them into the table below, starting with the pigment closest to the solvent front (top). Light green Pigment Rf values Pigments R/ value Suspected name of pigment 4.516.5 69 4.176.5 3.3/6.5 chlorophyll A chlorophy 1l A chlorojphyll B ,63 51 |3D
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