Compare the sizes of B. cereus, M. luteus, and R. rubrum cells (or their substitutes) as measured using a basic stain (Exercise 3-4) and an acidic stain. What might account for any differemce?

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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**Exercise 3: Comparing Cell Sizes with Staining Techniques**

**Task Description:**
Compare the sizes of *Bacillus cereus* (B. cereus), *Micrococcus luteus* (M. luteus), and *Rhodospirillum rubrum* (R. rubrum) cells—or their substitutes—using a basic stain (refer to Exercise 3-4) and an acidic stain. Consider what might account for any observed differences in size.

**Explanation:**

This exercise involves the use of two different types of stains to measure and compare the sizes of various bacterial cells. By using both a basic stain and an acidic stain, students are expected to observe potential differences in the appearance and size perception of the cells. These differences could be due to the distinct properties of the stains and how they interact with bacterial cell structures.

**Key Considerations:**
- **Basic Stains** are positively charged and bind easily to the negatively charged components of the bacterial cell, possibly affecting the observed size.
- **Acidic Stains** are negatively charged and typically stain the background, possibly offering a different perspective of cell size since they don't directly stain the cell.

This task helps students understand how staining techniques can influence the appearance and measurement of microscopic structures.
Transcribed Image Text:**Exercise 3: Comparing Cell Sizes with Staining Techniques** **Task Description:** Compare the sizes of *Bacillus cereus* (B. cereus), *Micrococcus luteus* (M. luteus), and *Rhodospirillum rubrum* (R. rubrum) cells—or their substitutes—using a basic stain (refer to Exercise 3-4) and an acidic stain. Consider what might account for any observed differences in size. **Explanation:** This exercise involves the use of two different types of stains to measure and compare the sizes of various bacterial cells. By using both a basic stain and an acidic stain, students are expected to observe potential differences in the appearance and size perception of the cells. These differences could be due to the distinct properties of the stains and how they interact with bacterial cell structures. **Key Considerations:** - **Basic Stains** are positively charged and bind easily to the negatively charged components of the bacterial cell, possibly affecting the observed size. - **Acidic Stains** are negatively charged and typically stain the background, possibly offering a different perspective of cell size since they don't directly stain the cell. This task helps students understand how staining techniques can influence the appearance and measurement of microscopic structures.
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