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compare the mechanisms of microbial death by dry and moist heat.
give 3 factors that would affect the thermal death time of bacterium.
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- Choose for the organisms below, depending on the incubation condition and media whether they will grow or not 1.Haemophilus influenzae in HBA CO2 2.Haemophilus influenzae in HBA anaerobic 3.Haemophilus influenzae in Chocolate agar CO2 4.Haemophilus influenzae in MacConkey 5.Clostridium perfringens in HBA CO2 6.Clostridium perfringens in HBA anaerobic 7.Clostridium perfringens in Chocolate agar CO2 8.Clostridium perfringens in MacConkey 9.Escherichia coli in HBA CO2 10.Escherichia coli in Chocolate agar CO2 11.Escherichia coli in MacConkey 12.Escherichia coli in HBA anaerobic.Identify two factors( in detail)that make biofilms more tolerant to antimicrobials including their mode of action.Discuss biofilms and their relevance to infectious diseases. Discuss population growth and the phases of a typical bacterial growth curve. Describe generation time for a bacterial population Explain how bacterial growth correlates to disease. Describe how environmental changes can alter the shape of a growth curve. Categorize microbes based on their preferred environmental niches (pH, temperature, oxygen and salt). Identify the biological properties that allow different classes of microbes to grow in extreme environments.
- explain how the use of an Eosin methylene blue agar plate can help determine the type of fermenters that bacteria are. explain throughly.List 5 temperature requirements for bacterial growth. Explain the temperature ranges and where do infectious bacteria fall in the temperature range and requirements.Identify two factors that make biofilms more tolerant to antimicrobials and make sure to include their mode of action.
- explain these types of microbial control methods: Refrigeration and freezing , Desiccation and Lyophilization, osmotic pressure, radiation and its effect to microorganisms.Please discuss the different environmental factors that influence microbial growth. What would be the basic profile of a microbe pathogenic to humans?Savlon Extra” disinfectant is known to exhibit good antimicrobial activity against Shigella dysenteriae. At 20oC a concentration of 5% v/v kills 2.0 x106 Sh. dysenteriae cells in 15 minutes. At 25oC the organisms were killed in 5 minutes. a) Calculate the temperature coefficient of Savlon Extra b). How long will it take the same concentration to kill the same number of Sh. dysenteriae cells at 30oC?
- Name four factors that affect the death rate and effectiveness of any microbial agent.Explain why gram negative bacteria in general are more toxic than gram positive bacteria.Choose one specific way from each of the two methods of inhibiting the growth of microbes below. Describe the method, explain the mechanism of action of killing the microorganisms and give practical applications of your chosen ways. 1. Physical Methods - Heat, cold, dessication, radiation, ultrasonic waves, filtration, gaseous atmosphere 2. Chemical Methods- disinfectants, antiseptics