Compare and contrast the operation of Optical microscopy and TEM in terms of Resolution

Human Anatomy & Physiology (11th Edition)
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Compare and contrast the operation of Optical microscopy and TEM in terms of Resolution

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Resolution of microscope:-  it is a ability to distinguish two tiny closer points or objects. It can be calculated by using the formula -

2dSinα =  λ

d = λ / 2Sinα

Here, 

d = minimum distance between two objects or points 

λ = wavelength of light or electron etc.

Sinα = angle of diffraction

Note:-

  • lower the value of "d", higher will be resolution.
  • Resolution increases as numerical aperture of lense increases.
  • Resolution increases as the wavelength of light source decreases.

 

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