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Colorimetric reagents. Proteins will intensely absorb 280 nm light even in the absence of a colorimetric reagent (See figure). Why is it advantageous to use the Bradford reagent to r protein concentration rather than just measuring protein absorbance (280 nm) directly?

Colorimetric reagents. Proteins will intensely absorb 280 nm light even in the absence of a colorimetric reagent (See figure). Why is it advantageous to use the Bradford reagent to r protein concentration rather than just measuring protein absorbance (280 nm) directly?

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Colorimetric reagents. Proteins will intensely absorb 280 nm light even in the absence of a colorimetric reagent (See figure). Why is it advantageous to use the Bradford reagent to r protein concentration rather than just measuring protein absorbance (280 nm) directly?
Transcribed Image Text:Colorimetric reagents. Proteins will intensely absorb 280 nm light even in the absence of a colorimetric reagent (See figure). Why is it advantageous to use the Bradford reagent to r protein concentration rather than just measuring protein absorbance (280 nm) directly?
Colorimetric reagents. Proteins will intensely absorb 280 nm light even in the absence of a colorimetric reagent (See figure). Why is it advantageous to use the Bradford reagent to measure protein concentration rather than just measuring protein absorbance (280 nm) directly?
Transcribed Image Text:Colorimetric reagents. Proteins will intensely absorb 280 nm light even in the absence of a colorimetric reagent (See figure). Why is it advantageous to use the Bradford reagent to measure protein concentration rather than just measuring protein absorbance (280 nm) directly?
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