Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Question
can you generate the unknown concentration using this information?
Transcribed Image Text:3 Section 9 Lowrys Method Excel Report
4
Absorbance
concentration OD at 720 Corrected
5 (μg/ml)
OD(nm)
(nm)
0
6
7
8
9
10
11
12
13
14 Unknown
15
16
17
BSA
19
88888
60
100
0.237
0.257
0.249
0.247
0.263
0.263
0
0.02
0.012
0.01
0.026
0.026
Raw Data Corrected
0.302 0.065
0.265
0.26
0255
8025
0.245
0.24
0.235
Absorbance OD at 720 (nm)
Determination of unknown BSA
Concentration by Lowry's Method
20
y 0.0002x 0 2422
-0.5819
40
60
NO
BSA concentration (pg/ml)
y 0,0002x+0.2422
0.065-0.0002x 0.2422
100
120
Transcribed Image Text:S.
No
1
2
3
4
5
6
7
BSA
Concentrat
ion
(ug/mL)
0
20
40
60
80
100
To be
Volume
of Stock
BSA (ML)
Blank
Distilled
Water
(mL)
1
.
0.2 200
0.4 00
0.6000
0.6600
0.4 400
0.8 800 02 200
1.01000
0
1.0 10000
0.8 0
Analytical
Reagent
(ml.)
light blue
2.5
2.5
2.5
2.5
2.5
2.5
Incubate
for 10
Temp.
1000, 1000, Boo
2.5
minutes
at Room
Folin's
Reagent
(mL)
0.5
0.5
0.5
0.5
0.5
0.5
0.5
Incuba
te for
37C
minutes
at Room
Temp.
Absorbance
(CD at
50-mm 130
0.331
0-261
0.249
0.263
0.263
0.300
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