Calculation % error (use Trial one) Sample ID Mass of Beaker and Empty test tube 2 ml 3 ml 4 ml 5 ml Trial 1 Trial 2 Trial 3 Average Sum of squares Variance Expected mass of 2 ml measurements (sum/n-1) Standard deviation measured liquid Square root of variance 2.0 g 3.0 g 4.0 g 5.0 g 9.52 g 9.52 g 9.52 g 9.52 g 2.12 1.94 2.41 2.16 Mass of beaker and test tube with measured liquid 11.64 g 12.52 g 17.95 g 14.72 g Measured Deviation Deviation value squared (value- mean) -0.04 -0.22 0.25 0.0016 0.0484 0.0625 Standard Deviation (use all three trials) noqon el agreVÀ You will need to do this for all measurements (2 ml, 3 ml, 4 ml and 5 ml) and for the comparison of pipeting with the transfer and eppendorf pipets. 0.1125 0.05625 0.237 Actual mass of liquid The average is reported as 2.16 g ± 0.237 2.12 g 2.98 g 3.96 g 5.17 g Difference between actual and expected 0.12 g 0.02 g 0.04 g 0.17 g Percent error 6% 0.7% 1% 3.4% fent

MATLAB: An Introduction with Applications
6th Edition
ISBN:9781119256830
Author:Amos Gilat
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How is percent error calculated ?
Calculation % error (use Trial one)
Sample ID
Mass of
Beaker and
Empty test
tube
2 ml
3 ml
4 ml
5 ml
Trial 1
Trial 2
Trial 3
Average
Sum of
2 ml measurements
squares
Variance
Expected
mass of
measured.
liquid
(sum/n-1)
Standard
deviation
2.0 g
3.0 g
Square
root of
variance
4.0 g
5.0 g
9.52 g
9.52 g
9.52 g
9.52 g
2.12
1.94
2.41
2.16
Mass of
beaker and
test tube
with
(value-
mean)
-0.04
-0.22
0.25
measured
liquid
11.64 g
12.52 g
17.95 g
14.72 g
Measured Deviation Deviation
value
squared
0.0016
0.0484
0.0625
0.1125
0.05625
Standard Deviation (use all three trials)
10.04880.0 2 banoqon alogenov
You will need to do this for all measurements (2 ml, 3 ml, 4 ml and 5 ml) and for the comparison
of pipeting with the transfer and eppendorf pipets.
0.237
Actual mass
of liquid
The average is reported as 2.16 g ± 0.237
2004
2.12 g
2.98 g
3.96 g
5.17 g
Difference
between
actual and
expected
0.12 g
0.02 g
0.04 g
0.17 g
Percent error
6%
0.7%
1%
3.4%
005
HISTA
DOLLIS
Transcribed Image Text:Calculation % error (use Trial one) Sample ID Mass of Beaker and Empty test tube 2 ml 3 ml 4 ml 5 ml Trial 1 Trial 2 Trial 3 Average Sum of 2 ml measurements squares Variance Expected mass of measured. liquid (sum/n-1) Standard deviation 2.0 g 3.0 g Square root of variance 4.0 g 5.0 g 9.52 g 9.52 g 9.52 g 9.52 g 2.12 1.94 2.41 2.16 Mass of beaker and test tube with (value- mean) -0.04 -0.22 0.25 measured liquid 11.64 g 12.52 g 17.95 g 14.72 g Measured Deviation Deviation value squared 0.0016 0.0484 0.0625 0.1125 0.05625 Standard Deviation (use all three trials) 10.04880.0 2 banoqon alogenov You will need to do this for all measurements (2 ml, 3 ml, 4 ml and 5 ml) and for the comparison of pipeting with the transfer and eppendorf pipets. 0.237 Actual mass of liquid The average is reported as 2.16 g ± 0.237 2004 2.12 g 2.98 g 3.96 g 5.17 g Difference between actual and expected 0.12 g 0.02 g 0.04 g 0.17 g Percent error 6% 0.7% 1% 3.4% 005 HISTA DOLLIS
8. Weigh each microfuge tube and record their mass in your lab notebook.
9. Calculate the mass of the liquid in each tube by subtracting the weight of the empty tube.
Results:
Note whether or not there is a difference in the accuracy of the different pipetting methods.
How would this influence which method you would use in class?
CALCULATIONS
Jest
Part 1: input the information in the table #1 for the initial 5 ml measurement: The percent
error is obtained by taking the difference between the actual mass and the expected mass of
the pipetted liquid and dividing it by the expected mass of the liquid. This is multiplied by
100 to give a percentage.
Table #1 - Calculation of percent error
Sample ID
dunia
2011
6. Press the bulb of a 1 ml graduated transfer pipet and lower the pipet into the beaker of
water. Gently release the bulb until the liquid passes the 0.5 ml mark on the pipet. Then
gently press the bulb down until the meniscus touches the 0.5 ml mark on the pipet to
measure 500 ul. Without releasing the bulb, lift the tranfer pipet out of the water.
Compare the volume of the water in each of the three tubes. Each tube should contain 500
ul of water.
7.
5 ml
Expected
mass of
measured
liquid
nizes
Mass of
Beaker and
Empty test
tube
Mass of
beaker and
test tube
with
measured
liquid
5.00g 127.46
Actual mass Difference
of liquid
between
actual and
brosse of
expected
gigerim
036
123.55
lilaca
Part 2: input the information in the table #2 for the all three of the 5 ml measurements: The
standard deviation is an analysis that determines the distribution of the data. If the values are
close together, this indicated a higher reproducibility and likelihood that a repeated
experiment will also have a value close to the mean. The variance from values to the mean is
calculated as the standard deviation. The standard deviations can help determine the level of
confidence you can have that data are reproducible.
To calculate the standard deviation perform the following steps:
Percent error
1. List the values and the mean
2. Subtract the mean from each value, which gives the deviation from the mean
3. Square each value (multiply it by itself)
4. Add all of these squares to obtain a total sum
22000
5. Divide the total sum by one less than the total number of values (n-1) this is the variance
6. Calculate the square root of the value; this is the standard deviation
Transcribed Image Text:8. Weigh each microfuge tube and record their mass in your lab notebook. 9. Calculate the mass of the liquid in each tube by subtracting the weight of the empty tube. Results: Note whether or not there is a difference in the accuracy of the different pipetting methods. How would this influence which method you would use in class? CALCULATIONS Jest Part 1: input the information in the table #1 for the initial 5 ml measurement: The percent error is obtained by taking the difference between the actual mass and the expected mass of the pipetted liquid and dividing it by the expected mass of the liquid. This is multiplied by 100 to give a percentage. Table #1 - Calculation of percent error Sample ID dunia 2011 6. Press the bulb of a 1 ml graduated transfer pipet and lower the pipet into the beaker of water. Gently release the bulb until the liquid passes the 0.5 ml mark on the pipet. Then gently press the bulb down until the meniscus touches the 0.5 ml mark on the pipet to measure 500 ul. Without releasing the bulb, lift the tranfer pipet out of the water. Compare the volume of the water in each of the three tubes. Each tube should contain 500 ul of water. 7. 5 ml Expected mass of measured liquid nizes Mass of Beaker and Empty test tube Mass of beaker and test tube with measured liquid 5.00g 127.46 Actual mass Difference of liquid between actual and brosse of expected gigerim 036 123.55 lilaca Part 2: input the information in the table #2 for the all three of the 5 ml measurements: The standard deviation is an analysis that determines the distribution of the data. If the values are close together, this indicated a higher reproducibility and likelihood that a repeated experiment will also have a value close to the mean. The variance from values to the mean is calculated as the standard deviation. The standard deviations can help determine the level of confidence you can have that data are reproducible. To calculate the standard deviation perform the following steps: Percent error 1. List the values and the mean 2. Subtract the mean from each value, which gives the deviation from the mean 3. Square each value (multiply it by itself) 4. Add all of these squares to obtain a total sum 22000 5. Divide the total sum by one less than the total number of values (n-1) this is the variance 6. Calculate the square root of the value; this is the standard deviation
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