Calculate the Rf of the spots from the samples. Report your answers in four decimal places.
Calculate the Rf of the spots from the samples. Report your answers in four decimal places.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Calculate the Rf of the spots from the samples. Report your answers in four decimal places.

Transcribed Image Text:Louie performed paper chromatography to identify which among the four available
reference mixtures – WAR, OFF, YIN, MEW – have the same components as his
unknown sample, GUN. He spotted the standards and the sample in a Whatman No. 1
filter paper, which he placed in a developing chamber containing 3% KCI. After 20
minutes, Louie obtained the chromatogram below, immediately noting that the distance
travelled by the solvent from the origin is 79.0 mm. On the other hand, the distances (in
cm) traveled by the developed spots were tabulated as follows.
solvent front
F
B
H
E
I
A
C
origin
WAR
OFF
ΥIN
MEW
GUN
DISTANCE
DISTANCE TRAVELLED FROM
SPOTS
TRAVELLED FROM
SPOTS
THE ORIGIN, cm
THE ORIGIN, cm
A
2.70
F
5.40
В
4.75
G
2.90
2.25
3.50
C
4.10
D
I
2.85
E
3.25
4.95
1. Is the type of paper chromatography performed normal or reversed? Why?
Expert Solution

This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 5 steps

Recommended textbooks for you

Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman

Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY

Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman

Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY

Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning

Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning

Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON