Calculate the relative amounts (in grams or milliliters) of each buffer component needed to prepare 1.50 L of 150 mM phosphate buffer pH 7.0 using a. NaH2PO4•6H2O (MM=228 g/mol) and Na2HPO4•6H2O (MM=250 g/mol) crystals. b. 500 mM NaH2PO4 and 750 mM Na2HPO4 stock solutions. c. NaH2PO4•6H2O crystals and 40% (w/v) NaOH (MM= 40 g/mol) solution. d. Na2HPO4•6H2O crystals and 10 M HCl solution. pKa values: pKa1=2.20, pKa2= 7.21, pKa3=12.70
Calculate the relative amounts (in grams or milliliters) of each buffer component needed to prepare 1.50 L of 150 mM phosphate buffer pH 7.0 using a. NaH2PO4•6H2O (MM=228 g/mol) and Na2HPO4•6H2O (MM=250 g/mol) crystals. b. 500 mM NaH2PO4 and 750 mM Na2HPO4 stock solutions. c. NaH2PO4•6H2O crystals and 40% (w/v) NaOH (MM= 40 g/mol) solution. d. Na2HPO4•6H2O crystals and 10 M HCl solution. pKa values: pKa1=2.20, pKa2= 7.21, pKa3=12.70
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Calculate the relative amounts (in grams or milliliters) of each buffer component needed to prepare
1.50 L of 150 mM phosphate buffer pH 7.0 using
a. NaH2PO4•6H2O (MM=228 g/mol) and Na2HPO4•6H2O (MM=250 g/mol) crystals.
b. 500 mM NaH2PO4 and 750 mM Na2HPO4 stock solutions.
c. NaH2PO4•6H2O crystals and 40% (w/v) NaOH (MM= 40 g/mol) solution.
d. Na2HPO4•6H2O crystals and 10 M HCl solution.
pKa values: pKa1=2.20, pKa2= 7.21, pKa3=12.70
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