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A biochemist prepares a buffer solution by adding enough TRIS and 12 M HCl to give 1.0 L of a buffer solution that has concentrations [TRIS] = 0.39 M and [TRISH*] = 0.56 M. (pKp of TRIS is 5.91.) (a) Calculate the pH of the buffer solution. pH = i (b) Suppose that 3.3 mL of 12 M HCI is added to 12 L of the buffer solution; calculate the new pH of the solution. pH = i

A biochemist prepares a buffer solution by adding enough TRIS and 12 M HCl to give 1.0 L of a buffer solution that has concentrations [TRIS] = 0.39 M and [TRISH*] = 0.56 M. (pKp of TRIS is 5.91.) (a) Calculate the pH of the buffer solution. pH = i (b) Suppose that 3.3 mL of 12 M HCI is added to 12 L of the buffer solution; calculate the new pH of the solution. pH = i

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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One of the most common buffers used in protein chemistry is a weak base called TRIS (the nitrogen atom of the amino group is the basic portion of the molecule): Но. -NH2 (HOCH2);CNH, = TRIS %3D A biochemist prepares a buffer solution by adding enough TRIS and 12 M HCI to give 1.0 L of a buffer solution that has concentrations [TRIS] = 0.39 M and [TRISH*] = 0.56 M. (pK, of TRIS is 5.91.) (a) Calculate the pH of the buffer solution. pH = i (b) Suppose that 3.3 mL of 12 M HCI is added to 12 L of the buffer solution; calculate the new pH of the solution. pH = i
Transcribed Image Text:One of the most common buffers used in protein chemistry is a weak base called TRIS (the nitrogen atom of the amino group is the basic portion of the molecule): Но. -NH2 (HOCH2);CNH, = TRIS %3D A biochemist prepares a buffer solution by adding enough TRIS and 12 M HCI to give 1.0 L of a buffer solution that has concentrations [TRIS] = 0.39 M and [TRISH*] = 0.56 M. (pK, of TRIS is 5.91.) (a) Calculate the pH of the buffer solution. pH = i (b) Suppose that 3.3 mL of 12 M HCI is added to 12 L of the buffer solution; calculate the new pH of the solution. pH = i
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