Calculate the 5 different volumes of BSA needed for the standard curve. Prepare a table showing the volume of BSA and water needed in the cuvette for each concnetration

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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Calculate the 5 different volumes of BSA needed for the standard curve. Prepare a table showing the volume of BSA and water needed in the cuvette for each concnetration

Prepare a standard curve as follows:
Pipette 1.0 ml of the dye reagent directly into cuvettes. Add increasing amounts of the protein
standard solution (BSA: 0.5 mg/ml) to the tubes from 5 µg to 25 ug in a total volume of 0.05 ml.
Transcribed Image Text:Prepare a standard curve as follows: Pipette 1.0 ml of the dye reagent directly into cuvettes. Add increasing amounts of the protein standard solution (BSA: 0.5 mg/ml) to the tubes from 5 µg to 25 ug in a total volume of 0.05 ml.
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